Thioredoxin Active Protein | yTXN1 active protein
Recombinant Yeast Thioredoxin
Gene Names
TRX1; LMA1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Synonyms
Thioredoxin; Recombinant Yeast Thioredoxin; TXN1 Yeast; Thioredoxin Yeast Recombinant; Thioredoxin-1; Thioredoxin I; TR-I; Thioredoxin-2; TRX1; TRX2; YLR043C; yTXN1 active protein
Host
E Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE.
Form/Format
Each mg of protein contains 20mM phosphate buffer pH 7.4.
Sterile Lyophilized Powder.
Sterile Lyophilized Powder.
Sequence Length
103
Solubility
It is recommended to reconstitute the lyophilized TRX in sterile 18M Omega -cm H2O.
Biological Activity
TRX activity is assayed by measuring the change in absorbance at 650 nm at 25 degree C using 0.13 uM bovine insulin containing 0.33mM DTT (pH 6.5).The specific activity was found to be 5.8units/mg.
Preparation and Storage
TRX although stable at 4 degree C for 3 weeks, should be stored desiccated below -18 degree C. Please prevent freeze thaw cycles.
Related Product Information for yTXN1 active protein
Description: Thioredoxin Yeast Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 12.6kDa.
Introduction: Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Introduction: Thioredoxins are small disulphide-containing redox proteins (within the conserved Cys-Gly-Pro-Cys active site) that have been found in all the kingdoms of living organisms. Thioredoxin contains a single disulfide active site and serves as a general protein disulphide oxidoreductase. Thioredoxins are involved in the first unique step in DNA synthesis. It interacts with a broad range of proteins by a redox mechanism based on reversible oxidation of two cysteine thiol groups to a disulphide, accompanied by the transfer of two electrons and two protons. The net result is the covalent interconversion of a disulphide and a dithiol. It has been suggested that thioredoxin may catalyze the formation of correct disulfides during protein folding because of its ability to act as an efficient oxidoreductant. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation.
Product Categories/Family for yTXN1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,235 Da
NCBI Official Full Name
thioredoxin TRX1
NCBI Official Symbol
TRX1
NCBI Official Synonym Symbols
LMA1
NCBI Protein Information
thioredoxin TRX1
UniProt Protein Name
Thioredoxin-1
Protein Family
UniProt Gene Name
TRX1
UniProt Synonym Gene Names
TRX2; TR-I
UniProt Entry Name
TRX1_YEAST
Uniprot Description
Participates as a hydrogen donor in redox reactions through the reversible oxidation of its active center dithiol to a disulfide, accompanied by the transfer of 2 electrons and 2 protons. It is involved in many cellular processes, including deoxyribonucleotide synthesis, repair of oxidatively damaged proteins, protein folding, sulfur metabolism, and redox homeostasis. Thioredoxin-dependent enzymes include phosphoadenosine-phosphosulfate reductase MET16, alkyl-hydroperoxide reductase DOT5, thioredoxin peroxidases TSA1 and TSA2, alkyl hydroperoxide reductase AHP1, and peroxiredoxin HYR1. Thioredoxin is also involved in protection against reducing stress. As part of the LMA1 complex, it is involved in the facilitation of vesicle fusion such as homotypic vacuole and ER-derived COPII vesicle fusion with the Golgi. This activity does not require the redox mechanism.
Research Articles on yTXN1
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Product Notes
The yTXN1 trx1 (Catalog #AAA142888) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thioredoxin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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