Thioredoxin-1 Active Protein | TRX1 active protein

Recombinant Thioredoxin-1

Cat. Num. AAA201132

• Gene Names: Txn1; ADF; Txn; Trx1; AW550880
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Thioredoxin-1; Recombinant Thioredoxin-1; TRX2; Thioredoxin I; TR-I; Thioredoxin-2; ADF; Surface associated sulphydryl protein; TXN protein; ATL derived factor; DKFZp686B1993; MGC61975; SASP; Thioredoxin; TRDX; TRX; TRX 1; TXN; TRX1 active protein

• Host: E Coli
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate-Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 1mg/ml (determined by BCA assay) (varies by lot)
• Sequence Positions: 1-105aa
• Sequence: MGSSHHHHHH SSGLVPRGSH MGS MVKLIES KEAFQEALAA AGDKLVVVDF SATWCGPCKM IKPFFHSLCD KYSNVVFLEV DVDDCQDVAA DCEVKCMPTF QFYKKGQKVG EFSGANKEKL EASITEYA
• Sequence Length: 105

• Applicable Applications for TRX1 active protein: SDS-PAGE, Enzyme Activity
• Species: Mouse
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Biological Activity: Specific activity is >60 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for TRX1 active protein

Thioredoxin-1 is a low molecular weight redox protein. Thioredoxin-1 contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Thioredoxin-1 also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant Mouse Thioredoxin-1 was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TRX1 active protein

Homeostasis; Protease inhibitors; Redox Proteins; Signal Transduction

References

Pigiet VP., et al.(1986) Proc. Natl.Acad.Sci. uSA 83(20):7643-7. Lundstrom J., et al. (1990) J.Biol.Chem. 265(16):9114-20.

NCBI and Uniprot Product Information

• NCBI GI #: 6755911
• NCBI GeneID: 22166
• NCBI Accession #: NP_035790.1
• NCBI GenBank Nucleotide #: NP_035790.1
• UniProt Accession #: P10639
• Molecular Weight: 14.1 kDa (128aa) confirmed by MALDI-TOF
• NCBI Official Full Name: thioredoxin
• NCBI Official Synonym Full Names: thioredoxin 1
• NCBI Official Symbol: Txn1
• NCBI Official Synonym Symbols: ADF; Txn; Trx1; AW550880
• NCBI Protein Information: thioredoxin
• UniProt Protein Name: Thioredoxin
• Protein Family: Thioredoxin
• UniProt Gene Name: Txn
• UniProt Synonym Gene Names: Txn1; Trx; ADF
• UniProt Entry Name: THIO_MOUSE

Uniprot Description

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.

Protein type: Nuclear receptor co-regulator

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; dendrite; extracellular region; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity; oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor

Biological Process: sulfate assimilation; response to radiation; negative regulation of protein export from nucleus; transcription, DNA-dependent; protein folding; regulation of transcription, DNA-dependent; cell redox homeostasis; transport; regulation of protein import into nucleus, translocation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; positive regulation of DNA binding