Hexokinase-1 Active Protein | HK-1 active protein

Recombinant Human Hexokinase-1

Cat. Num. AAA142772

• Gene Names: HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Hexokinase-1; Recombinant Human Hexokinase-1; HK1 Human; Hexokinase-1 Human Recombinant; EC 2.7.1.1; Hexokinase type I; HK I; Brain form hexokinase; HK1-ta; HK1-tb; HXK1; HK1; HK-1 active protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The protein (1mg/ml) contains 20mM Tris pH8.0 and 10% glycerol.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH GSGSQLFDHV AECLGDFMEK RKIKDKKLPV GFTFSFPCQQ SKIDEAILIT WTKRFKASGV EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT KDMLLEVKKR MRAEMELGLR KQTHNNAVVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA IKRREEFDLD VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISETLKTRG IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG AALITAVGVR LRTEASS
• Sequence Length: 917

• Biological Activity: Specific activity is >2 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 25C.
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Related Product Information for HK-1 active protein

Description: HK1 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain fused to 20 amino acids His tag at the N-terminal encoding the sequence of 937 amino acids and having a molecular mass of 104.6 kDa.HXK1 is purified by proprietary chromatographic techniques.

Introduction: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Hexokinase1 encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of HXK1 results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Product Categories/Family for HK-1 active protein

Protein Kinases; Enzymes; Hexokinase

NCBI and Uniprot Product Information

• NCBI GI #: 188497754
• NCBI GeneID: 3098
• NCBI Accession #: NP_000179.2
• NCBI GenBank Nucleotide #: NM_000188.2
• UniProt Accession #: P19367; O43443; O43444; O75574; Q5VTC3; Q96HC8; Q9NNZ4; Q9NNZ5; E9PCK0
• Molecular Weight: 101,085 Da
• NCBI Official Full Name: hexokinase-1 isoform HKI
• NCBI Official Synonym Full Names: hexokinase 1
• NCBI Official Symbol: HK1
• NCBI Official Synonym Symbols: HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
• NCBI Protein Information: hexokinase-1; HK I; brain form hexokinase; glycolytic enzyme; hexokinase type I
• UniProt Protein Name: Hexokinase-1
• Protein Family: Hexokinase
• UniProt Gene Name: HK1
• UniProt Synonym Gene Names: HK I
• UniProt Entry Name: HXK1_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Uniprot Description

HK1: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). HK-2 and its mitochondrial receptor (VDAC) play the most pivotal and direct roles in the ""Warburg effect"". Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Four human isoforms are produced by alternative splicing and alternative initiation. Isoform HK1 is markedly elevated in rapidly growing tumor cells exhibiting high glucose catabolic rates. HK1 is present in most tissues but is especially prominent in brain and kidney. Isoform HK1-SC is is an integral membrane protein detected in round spermatids, condensing spermatids and mature sperm where it is found in the head membranes, mitochondria of the midpiece and the fibrous sheath of the flagellum. Isoform HK1-SA is first expressed during meiosis and continues to be present in postmeiotic germ cells while isoform HK1-SB is present only in postmeiotic germ cells.

Protein type: EC 2.7.1.1; Carbohydrate Metabolism - starch and sucrose; Mitochondrial; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - galactose

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol; lipid raft

Molecular Function: hexokinase activity; protein binding; mannokinase activity; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; carbohydrate metabolic process; carbohydrate phosphorylation; glucose 6-phosphate metabolic process; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport

Disease: Hemolytic Anemia, Nonspherocytic, Due To Hexokinase Deficiency; Neuropathy, Hereditary Motor And Sensory, Russe Type