Ubiquitin carboxyl-terminal hydrolase 15 (Usp15) Recombinant Protein | Usp15 recombinant protein

Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 15 (Usp15) , partial

• Gene Names: Usp15; Gcap18; AI327321; 4921514G19Rik; E430033I05Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Ubiquitin carboxyl-terminal hydrolase 15 (Usp15); Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase 15 (Usp15); partial; Usp15 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 952

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 674651075
• NCBI GeneID: 14479
• NCBI Accession #: NP_001288557.1
• NCBI GenBank Nucleotide #: NM_001301628.1
• UniProt Accession #: Q8R5H1; Q3TGF5; Q3TTB2; Q3UL25; Q3UZH0; Q80TY6; Q80UK9
• Molecular Weight: 109,220 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 15 isoform 2
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 15
• NCBI Official Symbol: Usp15
• NCBI Official Synonym Symbols: Gcap18; AI327321; 4921514G19Rik; E430033I05Rik
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 15
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 15
• UniProt Gene Name: Usp15
• UniProt Synonym Gene Names: Kiaa0529

NCBI Description

The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.[provided by RefSeq, Aug 2014]

Uniprot Description

Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling, NF-kappa-B and RNF41/NRDP1-PRKN pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs (SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes. According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal. Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B. Acts as an inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes cleavage of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains attached by parkin on target proteins such as MFN2, thereby reducing parkin's ability to drive mitophagy. Acts as an associated component of COP9 signalosome complex (CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Involved in endosome organization by mediating deubiquitination of SQSTM1: ubiquitinated SQSTM1 forms a molecular bridge that restrains cognate vesicles in the perinuclear region and its deubiquitination releases target vesicles for fast transport into the cell periphery.