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Leptin Binding Domain Recombinant Protein

Recombinant Chicken Leptin Binding Domain

Purity
Greater than 99.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Synonyms
Leptin Binding Domain; Recombinant Chicken Leptin Binding Domain; Leptin Receptor Chicken; Leptin Receptor Chicken Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; Leptin Receptor; chLeptin Receptor; Leptin Binding Domain recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater than 99.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Form/Format
The protein was filter sterilized and stored at 4 degree C (0.2 to 0.5 mg/ml) solution of Tris-HCl buffer, pH 9.0 with 150mM NaCl.
Sterile Filtered colorless solution at a concentration of 0.4 mg/ml.
Sequence
The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-Asp-Val-Asn-IleBiological ActivityBiological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio
Protein Content
Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.
Preparation and Storage
Sterile solutions at 0.5mg/ml or less are stable at 4 degree C for several months.
Related Product Information for Leptin Binding Domain recombinant protein
Description: Leptin Binding Domain Chicken Recombinant also called Leptin Receptor produced in E Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa. Chicken Leptin Receptor consists of the cytokine binding domain of leptin receptor amino acids 420-626 of chicken leptin receptor.The Leptin Binding Domain is purified by proprietary chromatographic techniques.
Product Categories/Family for Leptin Binding Domain recombinant protein

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Product Notes

The Leptin Binding Domain (Catalog #AAA142385) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: The sequence of the first six N-terminal amino acids was determined and was found to be Ala-Ile-As p-Val-Asn- IleBiologi cal ActivityBi ological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio. It is sometimes possible for the material contained within the vial of "Leptin Binding Domain, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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