Stem Cell Factor Active Protein | rSCF active protein

Recombinant Rat Stem Cell Factor

Cat. Num. AAA142200

• Gene Names: Kitlg; Mgf; SCF; Kitl
• Purity: Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Stem Cell Factor; Recombinant Rat Stem Cell Factor; SCF Rat; Stem Cell Factor Rat Recombinant; Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250; Hematopoietic growth factor KL; rSCF active protein

• Host: E Coli
• Purity/Purification: Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

• Solubility: It is recommended to reconstitute the lyophilized SCF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED50 is determined by the dose-dependant stimulation of the proliferation of human TF-1 cells which is < 10 ng/ml, corresponding to a specific activity of 100,000units/mg.
• Preparation and Storage: Lyophilized rat SCF although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution SCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for rSCF active protein

Description: Stem cell factor Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids (26-189) and having a molecular mass of 18.4 kDa.The Rat SCF is purified by proprietary chromatographic techniques.

Introduction: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117 (c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

Product Categories/Family for rSCF active protein

Cytokines and Growth Factors; Growth Factors; Stem Cell Factor

NCBI and Uniprot Product Information

• NCBI GI #: 33667068
• NCBI GeneID: 60427
• NCBI Accession #: NP_068615.1
• NCBI GenBank Nucleotide #: NM_021843.4
• UniProt Accession #: P21581; Q9QWZ4; Q9Z2E7
• Molecular Weight: 27,681 Da
• NCBI Official Full Name: kit ligand isoform 1
• NCBI Official Synonym Full Names: KIT ligand
• NCBI Official Symbol: Kitlg
• NCBI Official Synonym Symbols: Mgf; SCF; Kitl
• NCBI Protein Information: kit ligand; C-kit ligand; hematopoietic growth factor KL; mast cell growth factor; steel factor/kit ligand; stem cell factor KL-1
• UniProt Protein Name: Kit ligand
• UniProt Gene Name: Kitlg
• UniProt Synonym Gene Names: Kitl; Mgf; MGF; SCF; sKITLG
• UniProt Entry Name: SCF_RAT

NCBI Description

soluble growth factor that activates c-kit tyrosine kinase receptor; important for signaling in spermatogenesis; putative regulator of Leydig cell development [RGD, Feb 2006]

Uniprot Description

SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane

Molecular Function: growth factor activity; cytokine activity; stem cell factor receptor binding

Biological Process: embryonic hemopoiesis; male gonad development; germ cell programmed cell death; positive regulation of MAP kinase activity; positive regulation of peptidyl-tyrosine phosphorylation; ovarian follicle development; positive regulation of melanocyte differentiation; negative regulation of mast cell apoptosis; positive regulation of cell proliferation; positive regulation of myeloid leukocyte differentiation; positive regulation of Ras protein signal transduction; cell adhesion; neural crest cell migration; positive regulation of DNA replication; negative regulation of apoptosis