AITRL recombinant protein

Recombinant Human AITRL, His Tag

Cat. Num. AAA142194

• Gene Names: TNFSF18; TL6; AITRL; GITRL; hGITRL
• Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: AITRL; Recombinant Human AITRL; His Tag; AITRL Human; His; AITRL Human Recombinant; Osteostat; TNFSF18; Activation-induced TNFR member Ligand; GITRL; TL6; Glucocorticoid-induced TNF-related ligand; hGITRL; Tumor necrosis factor ligand superfamily member 18; MGC138237; AITRL His; AITRL recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from 0.5 mg/ml in 0.03M Acetate buffer, pH-4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQLETAKEPC MAKFGPLPSK WQMASSEPPC VNKVSDWKLE ILQNGLYLIY GQVAPNANYN DVAPFEVRLY KNKDMIQTLT NKSKIQNVGG TYELHVGDTI DLIFNSEHQV LKNNTYWGII LIANPQEISL EHHHHHH
• Sequence Length: 199

• Solubility: Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited.
• Preparation and Storage: Lyophilized AITRL although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution AITRL should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for AITRL recombinant protein

Description: The Human AITRL His-Tagged Recombinant Protein, produced in E Coli, is 15.7 kDa (calculated) protein containing 125 amino acid residues of the human AITRL and 14 additional amino acid residues - HisTag (underlined). The amino acid sequence of the recombinant human Osteostat is homologous to the extracellular domain of the human TNF18, Thr53-Ser177.

Introduction: Osteostat is the cytokine that binds to TNFRSF18/AITR/GITR and is important for interactions between activated T-lymphocytes and endothelial cells and may modulate T-lymphocyte survival in peripheral tissues. Osteostat is expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells after stimulation by lipopolysaccharides.Osteostat protein is detectable in human microvascular EC and is highly up-regulated by IFN-alpha and IFN-beta. Osteostat inhibit differentiation of osteoclasts from monocytic precursor cells. Osteostat suppresses the early stage of osteoclastogenesis via inhibition of macrophage colony-stimulating factorinduced receptor activator of NF-kappaB (RANK) expression in the osteoclast precursor cells. Osteostat does not inhibit lipopolysaccharide-induced RANK expression in monocytes and dendritic cells, or activation-induced RANK expression in T cells. Osteostat is a novel regulator of osteoclast generation and substantiate the major role played by the endothelium in bone physiology.

Product Categories/Family for AITRL recombinant protein

Cytokines and Growth Factors; Cytokines; AITRL

NCBI and Uniprot Product Information

• NCBI GI #: 157419142
• NCBI GeneID: 8995
• NCBI Accession #: NP_005083.2
• NCBI GenBank Nucleotide #: NM_005092.3
• UniProt Accession #: Q9UNG2; O95852; Q6ISV1; A9IQG8
• Molecular Weight: 22,724 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 18
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 18
• NCBI Official Symbol: TNFSF18
• NCBI Official Synonym Symbols: TL6; AITRL; GITRL; hGITRL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 18; AITR ligand; GITR ligand; activation-inducible TNF-related ligand; glucocorticoid-induced TNF-related ligand; glucocorticoid-induced TNFR-related protein ligand
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 18
• UniProt Gene Name: TNFSF18
• UniProt Synonym Gene Names: AITRL; GITRL; TL6; AITRL; hGITRL
• UniProt Entry Name: TNF18_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF18: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T- cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: extracellular space; cell surface; plasma membrane; integral to membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding

Biological Process: tumor necrosis factor-mediated signaling pathway; cell-cell signaling; T cell proliferation during immune response; regulation of T cell proliferation; signal transduction; negative regulation of apoptosis; activation of NF-kappaB transcription factor