Human Tumor necrosis factor ligand superfamily member 18 ELISA Kit | TRAIL/TNFSF18 elisa kit

Human Tumor necrosis factor ligand superfamily member 18 ELISA Kit

Cat. Num. AAA761426

• Gene Names: TNFSF18; TL6; AITRL; GITRL; TNLG2A; hGITRL
• Reactivity: Human
• Synonyms: Tumor necrosis factor ligand superfamily member 18; Human Tumor necrosis factor ligand superfamily member 18 ELISA Kit; TNFSF18/GITRL/GITR Ligand/AITRL; TRAIL/TNFSF18 elisa kit

• Reactivity: Human
• Sequence Length: 199

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TRAIL/TNFSF18 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human TRAIL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human TRAIL antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TRAIL amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman TRAIL can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157419142
• NCBI GeneID: 8995
• NCBI Accession #: NP_005083.2
• NCBI GenBank Nucleotide #: NM_005092.3
• Molecular Weight: 22,724 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 18
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 18
• NCBI Official Symbol: TNFSF18
• NCBI Official Synonym Symbols: TL6; AITRL; GITRL; TNLG2A; hGITRL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 18
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 18
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF18
• UniProt Synonym Gene Names: AITRL; GITRL; TL6; AITRL; hGITRL
• UniProt Entry Name: TNF18_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFSF18: Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T- cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cytokine; Apoptosis

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: cell surface; extracellular space; integral to membrane; plasma membrane

Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor superfamily binding

Biological Process: activation of NF-kappaB transcription factor; adaptive immune response; cell-cell signaling; negative regulation of apoptosis; positive regulation of cell adhesion; positive regulation of inflammatory response; positive regulation of leukocyte migration; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of T cell proliferation; signal transduction; T cell proliferation during immune response; tumor necrosis factor-mediated signaling pathway