Keratinocyte Growth Factor-2 Active Protein | KGF 2 active protein

Recombinant Human Keratinocyte Growth Factor-2

Cat. Num. AAA142180

• Purity: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Synonyms: Keratinocyte Growth Factor-2; Recombinant Human Keratinocyte Growth Factor-2; KGF 2 Human; Keratinocyte Growth Factor-2 Human Recombinant; FGFA; FGF10; FGF-10; KGF-2; Fibroblast growth factor 10; KGF 2 active protein

• Host: E Coli
• Purity/Purification: Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
• Sequence Length: 208

• Solubility: It is recommended to reconstitute the lyophilized FGF-10 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.79 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of FGF-10 as a Reference Standard.
• Biological Activity: The ED50, calculated by the dose-dependant stimulation of FGF receptors by BaF3 indicator cells (measured by 3H-thymidine uptake) is < 0.5 ng/ml, corresponding to a specific activity of 2x106units/mg.
• Preparation and Storage: Lyophilized Keratinocyte Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF10 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for KGF 2 active protein

Description: Keratinocyte Growth Factor-2 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (40-208) and having a molecular mass of 19300 Dalton. Keratinocyte Growth Factor 2 is highly related to KGF-1(FGF-7), it binds to the same receptor as KGF-1 and shares 57% sequence homology. The FGF10 is purified by proprietary chromatographic techniques.

Introduction: KGF-2 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.

Product Categories/Family for KGF 2 active protein

Cytokines and Growth Factors; Growth Factors; Keratinocyte Growth Factor

NCBI and Uniprot Product Information

• NCBI GI #: 4758360
• NCBI GeneID: 2255
• NCBI Accession #: NP_004456.1
• NCBI GenBank Nucleotide #: NM_004465.1
• UniProt Accession #: O15520; Q6FHR3; Q6FHT6; Q96P59; C7FDY0
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• UniProt Protein Name: Fibroblast growth factor 10
• UniProt Gene Name: FGF10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Cytokine; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular matrix; extracellular space; cell surface; plasma membrane; extracellular region; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: salivary gland development; nerve growth factor receptor signaling pathway; activation of MAPK activity; urothelial cell proliferation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation involved in wound healing; muscle cell fate commitment; response to lipopolysaccharide; regulation of saliva secretion; embryonic pattern specification; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of fibroblast proliferation; epithelial cell proliferation; positive chemotaxis; embryonic digestive tract morphogenesis; induction of an organ; mesonephros development; embryonic genitalia morphogenesis; spleen development; positive regulation of keratinocyte migration; positive regulation of DNA repair; fibroblast growth factor receptor signaling pathway; positive regulation of urothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; metanephros development; positive regulation of epithelial cell proliferation; wound healing; radial glial cell differentiation; positive regulation of mitotic cell cycle; response to estradiol stimulus; positive regulation of vascular endothelial growth factor receptor signaling pathway; induction of positive chemotaxis; negative regulation of cell proliferation; establishment of mitotic spindle orientation; positive regulation of MAPKKK cascade; tissue regeneration; positive regulation of lymphocyte proliferation; pancreas development; male genitalia morphogenesis; thyroid gland development; lacrimal gland development; angiogenesis; otic vesicle formation; female genitalia morphogenesis; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; hair follicle morphogenesis; thymus development; keratinocyte proliferation; regulation of activin receptor signaling pathway; embryonic camera-type eye development; odontogenesis of dentine-containing teeth; limb bud formation; pituitary gland development; positive regulation of ATPase activity; actin cytoskeleton reorganization; white fat cell differentiation; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; positive regulation of Ras protein signal transduction; positive regulation of DNA replication; regulation of smoothened signaling pathway

Disease: Lacrimoauriculodentodigital Syndrome; Aplasia Of Lacrimal And Salivary Glands