Mouse BrdU Monoclonal Antibody | anti-BrdU antibody
Mouse Anti BrdU (5-bromo-2-deoxyuridine)
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A column
Synonyms
BrdU; Monoclonal Antibody; Mouse Anti BrdU (5-bromo-2-deoxyuridine); BrdU Antibody; BrdU (5-bromo-2-deoxyuridine); Mouse Antibody; anti-BrdU antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
YR-BrdU
Purity/Purification
Protein A column
Form/Format
1mg/ml in PBS (after reconstitution).
Applicable Applications for anti-BrdU antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
1:500- 1:2,000 (assay dependent)
Additional Information
This antibody was produced in BALB/c mice. This antibody can be used to detect proliferating cells in living tissues after incubation with BrdU which will be incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), in place of thymidine during DNA replication. Antibody binding requires prior denaturation of the DNA, by exposing the cells to acid or heat.
Reconstitution
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Immunogen
5-iodouridine covalently coupled to KLH.
Preparation and Storage
In lyophilized form, for long periods, store at 4 degree C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20 degree C.
Antibody is shipped lyophilized at ambient temperature.
Antibody is shipped lyophilized at ambient temperature.
Product Categories/Family for anti-BrdU antibody
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Product Notes
The BrdU (Catalog #AAA140391) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BrdU can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). 1:500- 1:2,000 (assay dependent). Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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