Mouse DnaJ Monoclonal Antibody | anti-DnaJ antibody

Mouse Anti Human DnaJ (HSP40)

Cat. Num. AAA140244

• Applications: ELISA, Western Blot
• Purity: DnaJ antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: DnaJ; Monoclonal Antibody; Mouse Anti Human DnaJ (HSP40); DnaJ Antibody; DnaJ (HSP40); Mouse Anti Human; HSP-40; HSP40; DNAJB1; HSPF1; Hdj1; DnaJ homolog subfamily B member 1; Heat shock 40 kDa protein 1; Heat shock protein 40; DnaJ protein homolog 1; HDJ-1; Sis1; anti-DnaJ antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a heavy chain and kappa light chain
• Clone Number: Pk1C7AT
• Purity/Purification: DnaJ antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.; Sterile Filtered colorless solution.
• Sequence Length: 370

• Applicable Applications for anti-DnaJ antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: DnaJ antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human DnaJ mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human DnaJ amino acids 1-340 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-DnaJ antibody

Introduction: Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK.

Product Categories/Family for anti-DnaJ antibody

Monoclonal Antibodies; Anti-Human Heat Shock Proteins; Monoclonal Antibodies; Anti Human Heat Shock Protein

NCBI and Uniprot Product Information

• NCBI GI #: 148506
• Molecular Weight: 40,331 Da
• NCBI Official Full Name: dnaJ
• UniProt Protein Name: Chaperone protein DnaJ
• Protein Family: DnaJ
• UniProt Gene Name: dnaJ
• UniProt Entry Name: DNAJ_ERYRH

Uniprot Description

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.