Mouse Dapper homolog 3 Monoclonal Antibody | anti-DACT3 antibody
Mouse Anti Human Dapper homolog 3
Gene Names
DACT3; RRR1; DAPPER3
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
DACT3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Dapper homolog 3; Monoclonal Antibody; Mouse Anti Human Dapper homolog 3; DACT3 Antibody; Mouse Anti Human; Antagonist of beta-catenin Dapper homolog 3; Arginine-rich region 1 protein; DACT3; RRR1; MGC15476; anti-DACT3 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 heavy chain and kappa light chain
Clone Number
P2A5AT
Purity/Purification
DACT3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sequence Length
629
Applicable Applications for anti-DACT3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
DACT3 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:250 ~ 1:500 and immunohistochemistry analysis is 1:50. Recommended starting dilution for Western blot is 1:250 and Immunohistochemistry is 1:50.
Immunogen
Anti-human DACT3 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human DACT3 amino acids 89-202 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-DACT3 antibody
Introduction: DACT3 which belongs to the DACT (Dpr/Frodo) gene family plays an imperative role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is linked to bivalent histone modifications.
Product Categories/Family for anti-DACT3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,949 Da
NCBI Official Full Name
Dapper homolog 3
NCBI Official Synonym Full Names
dishevelled-binding antagonist of beta-catenin 3
NCBI Official Symbol
DACT3
NCBI Official Synonym Symbols
RRR1; DAPPER3
NCBI Protein Information
dapper homolog 3; antagonist of beta-catenin Dapper homolog 3; arginine-rich region 1 protein; dapper, antagonist of beta-catenin, homolog 3
UniProt Protein Name
Dapper homolog 3
Protein Family
UniProt Gene Name
DACT3
UniProt Synonym Gene Names
RRR1
UniProt Entry Name
DACT3_HUMAN
Uniprot Description
DACT3: May be involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. Belongs to the dapper family.
Protein type: Unknown function
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: cytoplasm
Molecular Function: identical protein binding; protein kinase C binding; protein kinase A binding
Biological Process: Wnt receptor signaling pathway; negative regulation of Wnt receptor signaling pathway; negative regulation of cell growth
Research Articles on DACT3
Similar Products
Product Notes
The DACT3 dact3 (Catalog #AAA140243) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dapper homolog 3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). DACT3 antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:250 ~ 1:500 and immunohistochemistry analysis is 1:50. Recommended starting dilution for Western blot is 1:250 and Immunohistochemistry is 1:50. Researchers should empirically determine the suitability of the DACT3 dact3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dapper homolog 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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