Mouse Set7/9 Histone Methyltransferase Monoclonal Antibody | anti-SETD7 antibody

Mouse Anti Human Set7/9 Histone Methyltransferase

Cat. Num. AAA140201

• Gene Names: SETD7; KMT7; SET7; SET9; SET7/9
• Applications: ELISA, Western Blot
• Purity: SETD7 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Set7/9 Histone Methyltransferase; Monoclonal Antibody; Mouse Anti Human Set7/9 Histone Methyltransferase; SETD7 Antibody; Mouse Anti Human; Histone-lysine N-methyltransferase; H3 lysine-4 specific SET7; EC 2.1.1.43; Histone H3-K4 methyltransferase; H3-K4-HMTase; SET domain-containing protein 7; Set9; SET7/9; SETD7; anti-SETD7 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: Ps4E5AT
• Purity/Purification: SETD7 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 366

• Applicable Applications for anti-SETD7 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: SETD7 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human SETD7 mAb, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human SETD7 amino acids 1-366 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-SETD7 antibody

Introduction: Set 7/9 is a histone methyltransferase (HMTase) that transfers methyl groups to Lys4 of histone H3, in complex with S-adenosyl-L-methionine (AdoMet). The methylation of lysine residues of histones plays a critical role in the regulation of chromatin structure and gene expression. Acetylation, phosphorylation and methylation of the amino-terminal tails of histone are thought to be involved in the regulation of chromatin structure and function. The enzymes identified in the methylation of specific lysine residue on histones belong to the SET family with just one exception. Set7/9, unlike most other SET proteins, is exclusively a mono-methylase.

Product Categories/Family for anti-SETD7 antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 18139549
• NCBI GeneID: 80854
• NCBI Accession #: NP_085151.1
• NCBI GenBank Nucleotide #: NM_030648.2
• Molecular Weight: 40,721 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETD7
• NCBI Official Synonym Full Names: SET domain containing (lysine methyltransferase) 7
• NCBI Official Symbol: SETD7
• NCBI Official Synonym Symbols: KMT7; SET7; SET9; SET7/9
• NCBI Protein Information: histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; histone H3-lysine 4-specific methyltransferase; lysine N-methyltransferase 7
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD7
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD7
• UniProt Synonym Gene Names: KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
• UniProt Entry Name: SETD7_HUMAN

Uniprot Description

SETD7: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Interacts with IPF1/PDX-1. Widely expressed. Expressed in pancreatic islets. Belongs to the histone-lysine methyltransferase family. SET7 subfamily.

Protein type: EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: nucleoplasm; nucleolus; chromosome

Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; chromatin modification; response to DNA damage stimulus