Mouse Microsomal Glutathione S Transferase 1 (MGST1) ELISA Kit | MGST1 elisa kit

Mouse Microsomal Glutathione S Transferase 1 (MGST1) ELISA Kit

Cat. Num. AAA451222

• Reactivity: Mouse
• Synonyms: Microsomal Glutathione S Transferase 1 (MGST1); Mouse Microsomal Glutathione S Transferase 1 (MGST1) ELISA Kit; GST12; MGST-I; MGST1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MGST1. No significant cross-reactivity or interference between MGST1 and analogues was observed.
• Sequence Length: 155

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100U/L
• Sensitivity: 1U/L
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MGST1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MGST1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MGST1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MGST1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MGST1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MGST1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MGST1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MGST1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19705453
• NCBI GeneID: 171341
• NCBI Accession #: NP_599176.1
• NCBI GenBank Nucleotide #: NM_134349.3
• UniProt Accession #: P08011
• Molecular Weight: 17,472 Da
• NCBI Official Full Name: microsomal glutathione S-transferase 1
• NCBI Official Synonym Full Names: microsomal glutathione S-transferase 1
• NCBI Official Symbol: Mgst1
• NCBI Protein Information: microsomal glutathione S-transferase 1
• UniProt Protein Name: Microsomal glutathione S-transferase 1
• Protein Family: Microsomal glutathione S-transferase
• UniProt Gene Name: Mgst1
• UniProt Synonym Gene Names: Gst12; Microsomal GST-1

NCBI Description

enzyme that catalyzes the transfer of glutathione onto hydrophobic substrates [RGD, Feb 2006]

Uniprot Description

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.