Mouse Angiopoietin Like Protein 3 Monoclonal Antibody | anti-ANGPTL3 antibody

Mouse Anti Human Angiopoietin Like Protein 3

Cat. Num. AAA140164

• Gene Names: ANGPTL3; ANL3; ANG-5; FHBL2; ANGPT5
• Applications: ELISA, Western Blot
• Purity: ANGPTL3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Angiopoietin Like Protein 3; Monoclonal Antibody; Mouse Anti Human Angiopoietin Like Protein 3; ANGPTL3 Antibody; Mouse Anti Human; Angiopoietin 5; ANGPT5; ANGPTL3; anti-ANGPTL3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: P1D10AT
• Purity/Purification: ANGPTL3 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 460

• Applicable Applications for anti-ANGPTL3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ANGPTL3 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
• Immunogen: Anti-human ANGPTL3 mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with Recombinant human ANGPTL3 amino acids 243-460 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-ANGPTL3 antibody

Introduction: Angiopoietin-like proteins ANGPTL3 and ANGPTL4 are secreted proteins mainly expressed in liver that have been demonstrated to regulate triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. The experimental results show that Angptl3 and Angptl4 function to regulate circulating triglyceride levels during different nutritional states and therefore play a role in lipid metabolism during feeding/fasting through differential inhibition of Lipoprotein lipase (LPL). Using deletion mutants of Angiopoietin 5 human, it was demonstrated that the N-terminal domain containing fragment - (17207) and not the C-terminal fibrinogen-like domain containing fragment - (207460) increased the plasma triglyceride levels in mice.

Product Categories/Family for anti-ANGPTL3 antibody

Anti-Human CYTOKINES; Monoclonal Antibodies; Anti Human Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 7656888
• NCBI GeneID: 27329
• NCBI Accession #: NP_055310.1
• NCBI GenBank Nucleotide #: NM_014495.3
• Molecular Weight: 53,637 Da
• NCBI Official Full Name: angiopoietin-related protein 3 preproprotein
• NCBI Official Synonym Full Names: angiopoietin-like 3
• NCBI Official Symbol: ANGPTL3
• NCBI Official Synonym Symbols: ANL3; ANG-5; FHBL2; ANGPT5
• NCBI Protein Information: angiopoietin-related protein 3; angiopoietin 5
• UniProt Protein Name: Angiopoietin-related protein 3
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL3
• UniProt Synonym Gene Names: ANGPT5; ANG-5
• UniProt Entry Name: ANGL3_HUMAN

NCBI Description

This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metablism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Feb 2013]

Uniprot Description

ANGPTL3: Defects in ANGPTL3 are the cause of familial hypobetalipoproteinemia type 2 (FHBL2); also called combined hypobetalipoproteinemia familial. FHBL2 is a disorder of lipid metabolism characterized by less than 5th percentile age- and sex-specific levels of low density lipoproteins, and dietary fat malabsorption. Affected individuals present with combined hypolipidemia, consisting of extremely low plasma levels of LDL cholesterol, HDL cholesterol, and triglycerides.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: extracellular space; cell surface

Molecular Function: enzyme inhibitor activity; integrin binding; phospholipase inhibitor activity; growth factor activity

Biological Process: integrin-mediated signaling pathway; cholesterol metabolic process; glycerol metabolic process; phospholipid catabolic process; cell-matrix adhesion; lipid homeostasis; positive regulation of lipid catabolic process; signal transduction; fatty acid metabolic process; positive regulation of angiogenesis; cholesterol homeostasis; sequestering of lipid; acylglycerol homeostasis; phospholipid metabolic process; artery morphogenesis; negative regulation of lipoprotein lipase activity; phospholipid homeostasis; positive regulation of cell migration

Disease: Hypobetalipoproteinemia, Familial, 2