Replication protein A 70 kDa DNA-binding subunit (Rpa1) Recombinant Protein | Rpa1 recombinant protein

Recombinant Mouse Replication protein A 70 kDa DNA-binding subunit (Rpa1)

Cat. Num. AAA1387968

• Gene Names: Rpa1; Rpa; RF-A; RP-A; 70kDa; AA589576; AW557552; 5031405K23Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Replication protein A 70 kDa DNA-binding subunit (Rpa1); Recombinant Mouse Replication protein A 70 kDa DNA-binding subunit (Rpa1); Rpa1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-623, Full length protein
• Sequence: MVGHLSEGAIEVMIQQENTSIKPILQVINIRPISTGNRSPRYRLLMSDGLNTLSSFMLATQLNTLVEGGQLASNCVCQVHKFIVNTLKDGRKVVVLMDLEVMKSAEDVGLKIGNPVPYNEGYGQQQQQQQQQQQQAVPSPASAATPPASKPQPQNGSLGMGSTAAKAYGASKPFGKPAGTGLLQPSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGALKIANKQFSAVKNDYEMTFNNETSVLPCEDGHHLPTVQFDFTGIGDLESKAKDALVDIIGICKSYEDSIKITVKSNNREVAKRNIYLMDMSGKVVTTTLWGEDADKFDGSRQPVMAIKGARVSDFGGRSLSVLSSSTVIVNPDIPEAYKLRGWFDSEGQALDGVSISDHRSGGAGGGNTNWKTLHEAKSENLGQGDKADYFSTVAAVVFLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDREFPNFKYRMILSANIADFQENQWVTCFQESAEAILGQNTMYLGELKEKNEQAFEEVFQNANFRSFTFRIRVKLETYNDESRIKATVMDVKPVDFRDYGRRLIANIRKNM
• Sequence Length: 623

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 255982530
• NCBI GeneID: 68275
• NCBI Accession #: NP_001157695.1
• NCBI GenBank Nucleotide #: NM_001164223.1
• UniProt Accession #: Q8VEE4; Q3TEJ8
• Molecular Weight: 69,037 Da
• NCBI Official Full Name: replication protein A 70 kDa DNA-binding subunit isoform 1
• NCBI Official Synonym Full Names: replication protein A1
• NCBI Official Symbol: Rpa1
• NCBI Official Synonym Symbols: Rpa; RF-A; RP-A; 70kDa; AA589576; AW557552; 5031405K23Rik
• NCBI Protein Information: replication protein A 70 kDa DNA-binding subunit
• UniProt Protein Name: Replication protein A 70 kDa DNA-binding subunit
• Protein Family: Replication factor
• UniProt Gene Name: Rpa1
• UniProt Synonym Gene Names: RP-A p70; RF-A protein 1

Uniprot Description

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.