Immobilized aprotinin Protein | PTI protein
Immobilized bovine aprotinin
Gene Names
PTI; BPI; BPTI
Purity
>95% by SDS-PAGE analysis
Synonyms
Immobilized aprotinin; Immobilized bovine aprotinin; PTI protein
Host
Bovine lung
Purity/Purification
>95% by SDS-PAGE analysis
Form/Format
Resin
0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7.4
0.1M Tris-HCl; 0.15M NaCl; 0.02% Sodium Azide; pH 7.4
Sequence Length
100
Preservative
Sodium Azide
Preparation and Storage
Store at 4 degree C
Expiration: 12 months from delivery
Shipping: Cold pack
Expiration: 12 months from delivery
Shipping: Cold pack
Related Product Information for PTI protein
Immobilized bovine aprotinin, also known as trasylol or iniprol, is a reversible inhibitor of a range of serine proteases including trypsin, chymotrypsin, plasmin, and kallikrein. Binding occurs at a range of physiological conditions with elution of target proteases at very high or very low pH values. The agarose resin is easily removed by centrifugation or filtration and is reusable.
Immobilized Aprotinin Digestion Protocol
1. Make a digestion buffer consisting of 0.1M Tris-HCl, 0.15M NaCl, pH 7.4 (or other suitable physiological buffer such as PBS).
2. Wash desired amount of immobilized aprotinin with at least three column volumes of binding buffer.
3. Resuspend the washed immobilized aprotinin gel in binding buffer then add the resin to your protein sample.
4. Incubate mixture with gentle agitation for 30 minutes at room temperature.
5. Separate the immobilized aprotinin from the binding reaction by centrifugation or gravity filtration. Retain supernatant as your serine protease depleted protein sample.6. To elute bound active enzymes and regenerate immobilized aprotinin gel, thoroughly wash the resin with binding buffer then elute with 0.1M Glycine, 0.1M NaCl, pH 2.9. Collect elution into 1M Tris-HCl, pH 7.4 to restore neutral pH if desired.
Immobilized Aprotinin Digestion Protocol
1. Make a digestion buffer consisting of 0.1M Tris-HCl, 0.15M NaCl, pH 7.4 (or other suitable physiological buffer such as PBS).
2. Wash desired amount of immobilized aprotinin with at least three column volumes of binding buffer.
3. Resuspend the washed immobilized aprotinin gel in binding buffer then add the resin to your protein sample.
4. Incubate mixture with gentle agitation for 30 minutes at room temperature.
5. Separate the immobilized aprotinin from the binding reaction by centrifugation or gravity filtration. Retain supernatant as your serine protease depleted protein sample.6. To elute bound active enzymes and regenerate immobilized aprotinin gel, thoroughly wash the resin with binding buffer then elute with 0.1M Glycine, 0.1M NaCl, pH 2.9. Collect elution into 1M Tris-HCl, pH 7.4 to restore neutral pH if desired.
Product Categories/Family for PTI protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
6511
NCBI Official Full Name
Pancreatic trypsin inhibitor
NCBI Official Symbol
PTI
NCBI Official Synonym Symbols
BPI; BPTI
NCBI Protein Information
pancreatic trypsin inhibitor
UniProt Protein Name
Pancreatic trypsin inhibitor
UniProt Gene Name
BPI
UniProt Synonym Gene Names
BPTI
UniProt Entry Name
BPT1_BOVIN
Uniprot Description
Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
Research Articles on PTI
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Product Notes
The PTI bpi (Catalog #AAA136111) is a Protein produced from Bovine lung and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Immobilized aprotinin, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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