Human Apolipoprotein C3 ELISA Kit | APOC3 elisa kit

Human Apolipoprotein C3 ELISA Kit

Cat. Num. AAA136084

• Gene Names: APOC3; APOCIII
• Reactivity: Human
• Synonyms: Apolipoprotein C3; Human Apolipoprotein C3 ELISA Kit; APOC3 elisa kit

• Reactivity: Human
• Sequence Length: 99

• Epitope: Apolipoprotein C3
• Preparation and Storage: Store at 4 degree C.

Related Product Information for APOC3 elisa kit

Apolipoprotein C-III (ApoC3) is a 79 amino acid peptide synthesized in the liver and intestine and bound to the surface of triglyceride rich particles such as very low density lipoprotein and chylomicrons in plasma. ApoC3 controls the triglyceride composition of these particles by inhibiting lipoprotein lipase. ApoC3 loss-of-function mutations have been associated with markedly reduced levels of triglycerides and remnant cholesterol and a decrease in coronary-artery calcification, a surrogate marker for atherosclerosis. Therefore, ApoC3 is a potential new target for reducing residual cardiovascular risk. The sensitive quantitative measurement of total human ApoC3 antigen in plasma, serum, urine & other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The average concentration of ApoC3 in normal human plasma is 0.125 mg/ml. The assay measures total human ApoC3 in the 0.01-5 ng/ml range. Samples giving human ApoC3 levels above 5 ng/ml should be diluted in blocking buffer before use. A 1:200,000 to 1:1,000,000 dilution for normal human plasma is suggested for best results.
Human ApoC3 will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, biotinylated primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with horseradish peroxidase conjugated streptavidin. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ApoC3. The amount of color development is proportional to the concentration of total ApoC3 antigen in the sample.

Product Categories/Family for APOC3 elisa kit

Plasma Protein ELISA Kits; Human Kits

NCBI and Uniprot Product Information

• NCBI GI #: 4557323
• NCBI GeneID: 345
• NCBI Accession #: NP_000031.1
• NCBI GenBank Nucleotide #: NM_000040.3
• UniProt Accession #: P02656; Q08E83; Q6Q786
• Molecular Weight: 10,852 Da
• NCBI Official Full Name: apolipoprotein C-III
• NCBI Official Synonym Full Names: apolipoprotein C3
• NCBI Official Symbol: APOC3
• NCBI Official Synonym Symbols: APOCIII
• NCBI Protein Information: apolipoprotein C-III
• UniProt Protein Name: Apolipoprotein C-III
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC3
• UniProt Synonym Gene Names: Apo-CIII; ApoC-III

NCBI Description

This gene encodes a protein component of triglyceride (TG)-rich lipoproteins (TRLs) including very low density lipoproteins (VLDL), high density lipoproteins (HDL) and chylomicrons. The encoded protein plays a role in role in the metabolism of these TRLs through multiple modes. This protein has been shown to promote the secretion of VLDL1, inhibit lipoprotein lipase enzyme activity, and delay catabolism of TRL remnants. Mutations in this gene are associated with low plasma triglyceride levels and reduced risk of ischemic cardiovascular disease, and hyperalphalipoproteinemia, which is characterized by elevated levels of high density lipoprotein (HDL) and HDL cholesterol in human patients. This gene and other related genes comprise an apolipoprotein gene cluster on chromosome 11. [provided by RefSeq, Sep 2017]

Uniprot Description

Component of triglyceride-rich very low density lipoproteins (VLDL) and high density lipoproteins (HDL) in plasma (PubMed:18201179, PubMed:22510806). Plays a multifaceted role in triglyceride homeostasis (PubMed:18201179, PubMed:22510806). Intracellularly, promotes hepatic very low density lipoprotein 1 (VLDL1) assembly and secretion; extracellularly, attenuates hydrolysis and clearance of triglyceride-rich lipoproteins (TRLs) (PubMed:18201179, PubMed:22510806). Impairs the lipolysis of TRLs by inhibiting lipoprotein lipase and the hepatic uptake of TRLs by remnant receptors (PubMed:18201179, PubMed:22510806). Formed of several curved helices connected via semiflexible hinges, so that it can wrap tightly around the curved micelle surface and easily adapt to the different diameters of its natural binding partners (PubMed:18408013).