Jasmonic acid-amido synthetase JAR1 (JAR1) Recombinant Protein | JAR1 recombinant protein

Recombinant Arabidopsis thaliana Jasmonic acid-amido synthetase JAR1 (JAR1) , partial

• Gene Names: JAR1; AtGH3.11; F11C10.6; FAR-RED INSENSITIVE 219; FIN219; JASMONATE RESISTANT 1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Jasmonic acid-amido synthetase JAR1 (JAR1); Recombinant Arabidopsis thaliana Jasmonic acid-amido synthetase JAR1 (JAR1); partial; JAR1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 496

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 145331421
• NCBI GeneID: 819244
• NCBI Accession #: NP_001078069.1
• NCBI GenBank Nucleotide #: NM_001084600.1
• UniProt Accession #: Q9SKE2; Q42147; Q56YY1; Q949V9; Q9LKI2; C0Z3D1
• Molecular Weight: 55,195 Da
• NCBI Official Full Name: Auxin-responsive GH3 family protein
• NCBI Official Symbol: JAR1
• NCBI Official Synonym Symbols: AtGH3.11; F11C10.6; FAR-RED INSENSITIVE 219; FIN219; JASMONATE RESISTANT 1
• NCBI Protein Information: Auxin-responsive GH3 family protein
• UniProt Protein Name: Jasmonic acid-amido synthetase JAR1
• UniProt Gene Name: JAR1
• UniProt Synonym Gene Names: FIN219

NCBI Description

Encodes a jasmonate-amido synthetase that is a member of the GH3 family of proteins. JAR1 catalyzes the formation of a biologically active jasmonyl-isoleucine (JA-Ile) conjugate. JA-Ile promotes the interaction between JAZ1 and COI1 in the jasmonate signaling pathway. JAR1 localizes to the cytoplasm and is also a phytochrome A signaling component. JAR1 is an auxin-induced gene. Loss of function mutants are defective in a variety of responses to jasmonic acid. JAR1 has additional enzymatic activities in vitro, (e.g. the ability to synthesize adenosine 5'-tetraphosphate and other JA conjugates), but there are no data to show whether JAR1 catalyzes many of these reactions in vivo. JAR1 is involved in pathogen defense, sensitivity to ozone, and wound responses.

Uniprot Description

Catalyzes the synthesis of jasmonates-amino acid conjugates by adenylation; can use Ile and, in vitro at least, Val, Leu and Phe as conjugating amino acids on jasmonic acid (JA) and 9,10-dihydro-JA substrates, and to a lower extent, on 3-oxo-2-(2Z-pentenyl)-cyclopentane-1-butyric acid (OPC-4) and 12-hydroxy-JA (12-OH-JA). Can synthesize adenosine 5-tetraphosphate in vitro. Required for the JA-mediated signaling pathway that regulates many developmental and defense mechanisms, including growth root inhibition, vegetative storage proteins (VSPs) accumulation, induced systemic resistance (ISR), response to wounding and herbivores, tolerance to ozone O3 (probably having a role in lesion containment). Plays an important role in the accumulation of JA-Ile in response to wounding, both locally and systemically; promotes JA responding genes especially in distal part of wounded plants, via the JA-Ile-stimulated degradation of JAZ repressor proteins by the SCF(COI)E3 ubiquitin-protein ligase pathway. Involved in the apoptosis-like programmed cell death (PCD) induced by fungal toxin fumonisin B1-mediated (FB1). Required for volatile compounds (C6-aldehydes and allo-ocimene)-mediated defense activation. Involved in the non-pathogenic rhizobacterium-mediated ISR (defense priming) by P.fluorescens (strains CHAOr and WCS417r) and P.putida LSW17S against infection leaf pathogens such as P.syringae pv. tomato and H.parasitica. Required for the JA-dependent resistance to fungi such as P.irregulare, U.vignae and U.appendiculatus. Necessary to induce systemic resistance against R.solanaceraum and P.syringae pv. tomato with P.oligandrum (a non-pathogenic biocontrol agent) cell wall protein fraction (CWP). Mediates PGIP2 accumulation in response to B.cinerea infection and thus contributes to resistance against this pathogen. Modulates the UV-B alteration of leaves attractiveness to diamondback moths P.xylostella leading to insect oviposition. Involved in the regulation of far-red light influence on development. Seems necessary for the salicylic acid (SA)-mediated, NPR1-independent resistance pathway. May contribute to the chitin-elicited pathway. Contributes to the sensitivity toward F.graminearum.