PIWIL1 cdna clone
PIWIL1 cDNA Clone
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. The encoded protein may play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
PIWIL1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, peripheral
Chromosomal Location of Human Ortholog: 12q24.33
Cellular Component: cytoplasm
Molecular Function: mRNA binding; protein binding
Biological Process: RNA-mediated gene silencing; spermatid development
Research Articles on PIWIL1
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Product Notes
The PIWIL1 piwil1 (Catalog #AAA1277816) is a cDNA Clone and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: atgactggga gagcccgagc cagagccaga ggaagggccc gcggtcagga gacagcgcag ctggtgggct ccactgccag tcagcaacct ggttatattc agcctaggcc tcagccgcca ccagcagagg gggaattatt tggccgtgga cggcagagag gaacagcagg aggaacagcc aagtcacaag gactccagat atctgctgga tttcaggagt tatcgttagc agagagagga ggtcgtcgta gagattttca tgatcttggt gtgaatacaa ggcagaacct agaccatgtt aaagaatcaa aaacaggttc ttcaggcatt atagtaaggt taagcactaa ccatttccgg ctgacatccc gtccccagtg ggccttatat cagtatcaca ttgactataa cccactgatg gaagccagaa gactccgttc agctcttctt tttcaacacg aagatctaat tggaaagtgt catgcttttg atggaacgat attattttta cctaaaagac tacagcaaaa ggttactgaa gtttttagta agacccggaa tggagaggat gtgaggataa cgatcacttt aacaaatgaa cttccaccta catcaccaac ttgtttgcag ttctataata ttattttcag gaggcttttg aaaatcatga atttgcaaca aattggacga aattattata acccaaatga cccaattgat attccaagtc acaggttggt gatttggcct ggcttcacta cttccatcct tcagtatgaa aacagcatca tgctctgcac tgacgttagc cataaagtcc ttcgaagtga gactgttttg gatttcatgt tcaactttta tcatcagaca gaagaacata aatttcaaga acaagtttcc aaagaactaa taggtttagt tgttcttacc aagtataaca ataagacata cagagtggat gatattgact gggaccagaa tcccaagagc acctttaaga aagccgacgg ctctgaagtc agcttcttag aatactacag gaagcaatac aaccaagaga tcaccgactt gaagcagcct gtcttggtca gccagcccaa gagaaggcgg ggccctgggg ggacactgcc agggcctgcc atgctcattc ctgagctctg ctatcttaca ggtctaactg ataaaatgcg taatgatttt aacgtgatga aagacttagc cgttcataca agactaactc cagagcaaag gcagcgtgaa gtgggacgac tcattgatta cattcataaa aacgataatg ttcaaaggga gcttcgagac tggggtttga gctttgattc caacttactg tccttctcag gaagaatttt gcaaacagaa aagattcacc aaggtggaaa aacatttgat tacaatccac aatttgcaga ttggtccaaa gaaacaagag gtgcaccatt aattagtgtt aatccactag ataactggct gttgatctat acgcgaagaa attatgaagc agccaattca ttgatacaaa atctatttaa agttacacca gccatgggca tgcaaatgaa aaaagcaata atgattgaag tggatgacag aactgaagcc tacttaagag tcttacagca aaaggtcaca gcagacaccc agatagttgt ctgtctgttg tcaagtaatc ggaaggacaa atacgatgct attaaaaaat acccgtgtac agattgccct accccaagtc agtgtgtggt ggcccgaacc ttaggcaaac agcaaactgt catggccatt gctacaaaga ttgccctaca gatgaactgc aagatgggag gagagctctg gagggtggac atccccctga agctcgtgat gatcgttggc atcgattgtt accatgacat gacagctggg cggaggtcaa tcgcaggatt tgttgccagc atcaatgaag ggatgacccg ctggttctca cgctgcatat ttcaggatag aggacaggag ctggtagatg ggctcaaagt ctgcctgcaa gcggctctga gggcttggaa tagctgcaat gagtacatgc ccagccggat catcgtgtac cgcgatggcg taggagacgg ccagctgaaa acactggtga actacgaagt gccacagttt ttggattgtc taaaatccat tggtagaggt tacaacccta gactaacggt aattgtggtg aagaaaagag tgaacaccag attttttgct cagtctggag gaagacttca gaatccactt cctggaacag ttattgatgt agaggttacc agaccagaat ggtatgactt ttttatcgtg agccaggctg tgagaagtgg tagtgtttct cccacacatt acaatgtcat ctatgacaac agcggcctga agccagacca catacagcgc ttgacctaca agctgtgcca catctattac aactggccag gtgtcattcg tgttcctgct ccttgccagt acgcccacaa gctggctttt cttgttggcc agagtattca cagagagcca aatctgtcac tgtcaaaccg cctttactac ctctaa. It is sometimes possible for the material contained within the vial of "PIWIL1, cDNA Clone" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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