Typical Testing Data/Standard Curve (for reference only)
Human PDGFRbeta ELISA Kit | PDGFRbeta elisa kit
Human PDGFRbeta (Platelet Derived Growth Factor Receptor Beta) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PDGFRbeta. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PDGFRbeta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PDGFRbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PDGFRbeta. You can calculate the concentration of Human PDGFRbeta in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
PDGFRB: a receptor tyrosine kinase of the PDGFR family that binds members of the platelet-derived growth factor family. The identity of the growth factor bound determines whether the functional receptor is a homodimer or a heterodimer, composed of both PDGFR-alpha and -beta. Ligand binding induces receptor dimerization and autophosphorylation, thereby recruiting SH2-containing proteins including Grb2, Src, GAP, PTPN11, PI3 kinase, PLC-gamma and Nck. Regulates cell growth, actin reorganization, migration and differentiation. A variety of myeloproliferative disorders and cancers result from translocations that activate PDGFRbeta by fusion with proteins such as TEL/ETV6, H2, CEV14/TRP11, rabaptin 5, and huntington interacting protein 1. Gleevec treatment of TEL fusions has been successful. Overexpressed in metastatic medulloblastoma. Inhibitors: Gleevec, Sutent.
Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Oncoprotein; Protein kinase, TK; EC 2.7.10.1; Kinase, protein; TK group; PDGFR family
Cellular Component: focal adhesion; cell surface; membrane; lysosome; cytoplasm; apical plasma membrane; integral to membrane; plasma membrane; cytoplasmic vesicle; nucleus; intrinsic to plasma membrane
Molecular Function: platelet-derived growth factor binding; nucleotide binding; platelet-derived growth factor receptor binding; transmembrane receptor protein tyrosine kinase activity; platelet-derived growth factor receptor activity; protein kinase binding; protein kinase activity; transferase activity; signal transducer activity; protein binding; protein-tyrosine kinase activity; platelet-derived growth factor beta-receptor activity; transferase activity, transferring phosphorus-containing groups; phosphoinositide 3-kinase binding; kinase activity; ATP binding; receptor binding
Biological Process: positive regulation of phosphoprotein phosphatase activity; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; multicellular organismal development; positive regulation of smooth muscle cell proliferation; positive regulation of collagen biosynthetic process; cardiac myofibril assembly; platelet-derived growth factor receptor signaling pathway; signal transduction; positive regulation of smooth muscle cell migration; chemotaxis; protein amino acid phosphorylation; smooth muscle development; positive regulation of MAP kinase activity; nitrogen compound metabolic process; positive regulation of cell proliferation; tissue homeostasis; hemopoiesis; phosphatidylinositol metabolic process; kidney development; regulation of peptidyl-tyrosine phosphorylation; cell migration; phosphoinositide-mediated signaling; positive regulation of mitosis; positive regulation of chemotaxis; adrenal gland development; in utero embryonic development; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; glycosaminoglycan biosynthetic process; skeletal morphogenesis; regulation of actin cytoskeleton organization and biogenesis; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration; negative regulation of apoptosis
Research Articles on PDGFRbeta
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Product Notes
The Human PDGFRbeta pdgfrb (Catalog #AAA2503541) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2503541 ELISA Kit recognizes Human PDGFRbeta. It is sometimes possible for the material contained within the vial of "PDGFRbeta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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