Rabbit anti-Human ABCB1 Polyclonal Antibody | anti-ABCB1 antibody

ABCB1 Polyclonal Antibody

Cat. Num. AAA126773

• Gene Names: ABCB1; CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ABCB1; Polyclonal Antibody; ABCB1 Polyclonal Antibody; Multidrug resistance protein 1; MDR1; PGY1; anti-ABCB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1280

• Applicable Applications for anti-ABCB1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western Blot (WB): 1:200-1:500; Immunohistochemistry (IHC): 1:50-1:100
• Immunogen: A synthetic peptide of human ABCB1
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ABCB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Related Product Information for anti-ABCB1 antibody

MDR1/ABCB1 belongs to the Mdr/Tap subfamily of the ATP binding casette transporter superfamily (1). MDR1 serves as an efflux pump for xenobiotic compounds with broad substrate specificity. Its substrates include therapeutic agents, such as actinomycin D, etoposide, imatinib, and doxorubicin, as well as endogenous molecules, such as betaamyloids, steroid hormones, lipids, phospholipids, cholesterol, and cytokines (2). Research studies have shown that MDR1 reduces drug accumulation in cancer cells, allowing the development of drug resistance (35). On the other hand, MDR1 expressed in the plasma membrane of cells in the bloodbrain, bloodcerebral spinal fluid, or bloodplacenta barriers restricts the permeability of drugs into these organs from the apical or serosal side (6,7). MDR1 is also expressed in normal tissues with excretory function such as small intestine, liver, and kidney (7). Intracellular MDR1 has been detected in the ER, vesicles, and nuclear envelope, and has been associated with cell trafficking machinery (8). Other reported functions of MDR1 include viral resistance, cytokine trafficking (9,10), and lipid homeostasis in the peripheral and central nervous system (1113).

References

1. Furuya, K.N. et al. (1997) Cancer Res 57, 3708-16 2. Litman, T. et al. (1997) Biochim Biophys Acta 1361, 16976. 3. Chen, C.J. et al. (1986) Cell 47, 3819. 4. Kartner, N. et al. (1983) Cancer Res 43, 44139. 5. Chen, G. et al. (1997) J Biol Chem 272, 597482. 6. Brinkmann, U. and Eichelbaum, M. (2001) Pharmacogenomics J 1, 5964. 7. Fromm, M.F. (2004) Trends Pharmacol Sci 25, 4239. 8. Miller, D.S. et al. (2008) Pharmacol Rev 60, 196209. Ambudkar, S.V. et al. (1999) Annu Rev Pharmacol Toxicol 39, 36198. 10. Raviv, Y. et al. (2000) FASEB J 14, 5115 11. Meijer, O.C. et al. (2003) J Endocrinol 178, 138. 12. Karssen, A.M. et al. (2002) J Endocrinol 175, 25160 13. Jeannesson, E. et al. (2009) Clin Chim Acta 403, 198202.

NCBI and Uniprot Product Information

• NCBI GI #: 42741659
• NCBI GeneID: 5243
• NCBI Accession #: NP_000918.2
• NCBI GenBank Nucleotide #: NM_000927.4
• UniProt Accession #: P08183; Q12755; Q14812; A8K294
• Molecular Weight: 141kDa
• NCBI Official Full Name: multidrug resistance protein 1
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• NCBI Official Symbol: ABCB1
• NCBI Official Synonym Symbols: CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
• NCBI Protein Information: multidrug resistance protein 1; P-glycoprotein 1; colchicin sensitivity; doxorubicin resistance
• UniProt Protein Name: Multidrug resistance protein 1
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB1
• UniProt Synonym Gene Names: MDR1; PGY1
• UniProt Entry Name: MDR1_HUMAN