Typical Testing Data/Standard Curve (for reference only)
Bovine Serum Albumin ELISA Kit | SA elisa kit
Bovine Serum Albumin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SALB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SALB antibody and an SALB-HRP conjugate. The assay sample and buffer are incubated together with SALB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SALB concentration since SALB from samples and SALB-HRP conjugate compete for the anti-SALB antibody binding site. Since the number of sites is limited, as more sites are occupied by SALB from the sample, fewer sites are left to bind SALB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SALB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]
Uniprot Description
albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carrier; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus
Molecular Function: antioxidant activity; toxin binding; protein binding; enzyme binding; copper ion binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding
Biological Process: platelet activation; receptor-mediated endocytosis; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; hemolysis by symbiont of host red blood cells; lipoprotein metabolic process; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; positive regulation of circadian sleep/wake cycle, non-REM sleep; transmembrane transport; response to nutrient; negative regulation of apoptosis
Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic
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Product Notes
The Bovine SA alb (Catalog #AAA733959) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733959 ELISA Kit recognizes Bovine SA. It is sometimes possible for the material contained within the vial of "Serum Albumin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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