Mitochondrial intermembrane space import and assembly protein 40 (MIA40) Recombinant Protein | MIA40 recombinant protein

Recombinant Saccharomyces cerevisiae Mitochondrial intermembrane space import and assembly protein 40 (MIA40)

• Gene Names: MIA40; FMP15; TIM40
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Mitochondrial intermembrane space import and assembly protein 40 (MIA40); Recombinant Saccharomyces cerevisiae Mitochondrial intermembrane space import and assembly protein 40 (MIA40); Recombinant Mitochondrial intermembrane space import and assembly protein 40 (MIA40); Mitochondrial intermembrane space import and assembly protein 40; Mitochondrial import inner membrane translocase TIM40; MIA40 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 32-403
• Sequence: MASSPQFGRNSNQEKTAGFIMGILSMAGALYFIAPNRKPLFASRKVESDKTAEEELSSGGEQSPENEDDNNSKSDENGDDNDSKNDETEAGPQLGGDKIGASKVAEDGELVVLAEEDNKSSEDKDTDESKVSTKDDEQSNEDNATANNQKDENISSENSEENTSDKTLDNNAGSSEKKDPEHSDDEKSQQGQSDDKTTTEDNNGEEESSKKTVSDSENSAKQSESSDEEKEELRKQEEKQMGPTEEEVQHEGAYNPDTGEINWDCPCLGGMAHGPCGEEFKSAFSCFVYSEAEPKGIDCVEKFQHMQDCFRKYPEHYAEQLKETSDDEEPQDKVKVNTIESAPNVSSAKENAAKKAEQSDVKKEPLNEESKP
• Sequence Length: 403

• Species: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 37362671
• NCBI GeneID: 853639
• NCBI Accession #: NP_012726.2
• NCBI GenBank Nucleotide #: NM_001179761.1
• UniProt Accession #: P36046; D6VX05
• Molecular Weight: 44,536 Da
• NCBI Official Full Name: Mia40p
• NCBI Official Symbol: MIA40
• NCBI Official Synonym Symbols: FMP15; TIM40
• NCBI Protein Information: Mia40p
• UniProt Protein Name: Mitochondrial intermembrane space import and assembly protein 40
• Protein Family: Mitochondrial intermembrane space import and assembly protein
• UniProt Gene Name: MIA40
• UniProt Synonym Gene Names: TIM40
• UniProt Entry Name: MIA40_YEAST

Uniprot Description

Function: Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS). Forms a redox cycle with ERV1 that involves a disulfide relay system. Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced MIA40 is reoxidized by FAD-linked sulfhydryl oxidase ERV1. Ref.3 Ref.8 Ref.9 Ref.10 Ref.11

Cofactor: Copper or zinc.

Subunit structure: Monomer. Interacts with the FAD-linked sulfhydryl oxidase ERV1 and with the substrate proteins COX17, TIM9, and TIM13, forming transient intermolecular disulfide bridges. Interacts with FCJ1. Ref.3 Ref.8 Ref.10 Ref.11 Ref.14

Subcellular location: Mitochondrion inner membrane; Single-pass type II membrane protein; Intermembrane side Ref.3 Ref.5 Ref.7 Ref.8 Ref.9 Ref.12.

Domain: The CHCH domain contains a conserved twin Cys-X(9)-Cys motif which is required for import and stability of MIA40 in mitochondria

Probable.

Miscellaneous: Present with 5040 molecules/cell in log phase SD medium.

Sequence similarities: Contains 1 CHCH domain.