Chemotaxis protein CheY (cheY) Recombinant Protein | cheY recombinant protein

Recombinant Escherichia coli Chemotaxis protein CheY (cheY)

Cat. Num. AAA1205632

• Gene Names: cheY; ECK1883; JW1871
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Chemotaxis protein CheY (cheY); Recombinant Escherichia coli Chemotaxis protein CheY (cheY); cheY recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-129, full length protein
• Sequence: ADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM
• Sequence Length: 128

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16129834
• NCBI GeneID: 946393
• NCBI Accession #: NP_416396.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AE67; P06143
• Molecular Weight: 14,097 Da
• NCBI Official Full Name: chemotaxis regulator transmitting signal to flagellar motor component
• NCBI Official Symbol: cheY
• NCBI Official Synonym Symbols: ECK1883; JW1871
• NCBI Protein Information: chemotaxis regulator transmitting signal to flagellar motor component
• UniProt Protein Name: Chemotaxis protein CheY
• Protein Family: Chemotaxis protein
• UniProt Gene Name: cheY

NCBI Description

CheY is acetylated at lysine residues 92 and 109 by acetyl-CoA synthase (Acs). [More information is available at EcoGene: EG10150]. Chemotaxis regulator protein CheY is activated through phosphorylation of an aspartate residue by the CheA(L)-his-P protein. [More information is available at EcoCyc: EG10150].

Uniprot Description

Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.