Phosphomethylpyrimidine synthase (thiC) Recombinant Protein | Swit_3201 recombinant protein
Recombinant Sphingomonas wittichii Phosphomethylpyrimidine synthase (thiC)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Phosphomethylpyrimidine synthase (thiC); Recombinant Sphingomonas wittichii Phosphomethylpyrimidine synthase (thiC); Recombinant Phosphomethylpyrimidine synthase (thiC); Phosphomethylpyrimidine synthase EC= 4.1.99.17; Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase Thiamine biosynthesis protein ThiC; Swit_3201 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-619
Sequence
MADIPARTEMKVTTGPIRGSRKIHVEGPQGVRVAMREIALEPSSGEPPVRVYDCSGPYTDPNAHIDIMAGLPALRRDWILGRGDVEDYEGRAIKPEDNGLKGPDRSGGVTPFPNVVRRPLRAKAGQNVSQMHYARRGIITPEMEYVAIRENVGRAALKEKLLRDGEDFGAAIPDFVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVAAEVDKMVWSIRWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGIAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVRLPFIPMTAKRVTGIVSRGGSIMAKWCLAHHRESFLYERFDEICEIMKAYDVAFSLGDGLRPGSIADANDEAQFSELKTLGELTQIAWQHDVQVMIEGPGHVPMHKIKANMDKQLEACGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAKLRDDALSRARFEFRWRDQFNLSLDPDTAEQYHDQTLPAEGAKTAHFCSMCGPKFCSMKITQEVRDFAATRNAPADQFIAAGDAEAGMRAMSDVFREKGGEIYLPAAE
Sequence Length
619
Species
Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
68,116 Da
NCBI Official Full Name
thiamine biosynthesis protein ThiC
NCBI Official Symbol
Swit_3201
NCBI Protein Information
thiamine biosynthesis protein ThiC
UniProt Protein Name
Phosphomethylpyrimidine synthase
UniProt Gene Name
thiC
UniProt Synonym Gene Names
HMP-P synthase; HMP-phosphate synthase
UniProt Entry Name
THIC_SPHWW
Uniprot Description
Function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction
By similarity. HAMAP-Rule MF_00089
Catalytic activity: 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-phosphomethylpyrimidine + 5'-deoxyadenosine + L-methionine + formate + CO. HAMAP-Rule MF_00089
Cofactor: Binds 1 4Fe-4S cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine
By similarity.
Pathway: Cofactor biosynthesis; thiamine diphosphate biosynthesis. HAMAP-Rule MF_00089
Subunit structure: Homodimer
By similarity. HAMAP-Rule MF_00089
Sequence similarities: Belongs to the ThiC family.
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Product Notes
The Swit_3201 thic (Catalog #AAA1200739) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-619. The amino acid sequence is listed below: MADIPARTEM KVTTGPIRGS RKIHVEGPQG VRVAMREIAL EPSSGEPPVR VYDCSGPYTD PNAHIDIMAG LPALRRDWIL GRGDVEDYEG RAIKPEDNGL KGPDRSGGVT PFPNVVRRPL RAKAGQNVSQ MHYARRGIIT PEMEYVAIRE NVGRAALKEK LLRDGEDFGA AIPDFVTPEF VRDEVARGRA IIPSNINHPE SEPMAIGRNF LVKINANIGN SAVASDVAAE VDKMVWSIRW GADTVMDLST GRNIHDTREW ILRNSPVPIG TVPIYQALEK VGGIAEDLTW EIFRDTLIEQ AEQGVDYFTI HAGVRLPFIP MTAKRVTGIV SRGGSIMAKW CLAHHRESFL YERFDEICEI MKAYDVAFSL GDGLRPGSIA DANDEAQFSE LKTLGELTQI AWQHDVQVMI EGPGHVPMHK IKANMDKQLE ACGEAPFYTL GPLTTDIAPG YDHITSAIGA AMIGWFGTAM LCYVTPKEHL GLPDRDDVKV GVVTYKLAAH AADLAKGHPA AKLRDDALSR ARFEFRWRDQ FNLSLDPDTA EQYHDQTLPA EGAKTAHFCS MCGPKFCSMK ITQEVRDFAA TRNAPADQFI AAGDAEAGMR AMSDVFREKG GEIYLPAAE. It is sometimes possible for the material contained within the vial of "Phosphomethylpyrimidine synthase (thiC), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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