Preprotein translocase subunit SecE (secE) Recombinant Protein | secE recombinant protein

Recombinant Escherichia coli Preprotein translocase subunit SecE (secE)

• Gene Names: secE; ECK3972; JW3944; mbrC; prlG
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Preprotein translocase subunit SecE (secE); Recombinant Escherichia coli Preprotein translocase subunit SecE (secE); Recombinant Preprotein translocase subunit SecE (secE); Preprotein translocase subunit SecE; secE recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-127
• Sequence: MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLRALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTAVMSLILWGLDGILVRLVSFITGLRF
• Sequence Length: 127

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 16131811
• NCBI GeneID: 948486
• NCBI Accession #: NP_418408.1
• NCBI GenBank Nucleotide #: NC_000913.2
• UniProt Accession #: P0AG96; P16920; Q2M8R7
• Molecular Weight: 13,643 Da
• NCBI Official Full Name: preprotein translocase membrane subunit
• NCBI Official Symbol: secE
• NCBI Official Synonym Symbols: ECK3972; JW3944; mbrC; prlG
• NCBI Protein Information: preprotein translocase membrane subunit
• UniProt Protein Name: Preprotein translocase subunit SecE
• Protein Family: Protein translocase
• UniProt Gene Name: secE
• UniProt Synonym Gene Names: prlG
• UniProt Entry Name: SECE_ECOLI

NCBI Description

SecYEG complex interacts with SecDFyajC to form a hexameric holocomplex. mbrC17 is a point mutation 23 nt before secE that increases secE and nusB expression. [More information is available at EcoGene: EG10939]. SecE is an inner membrane protein involved in the Sec secretion pathway. [More information is available at EcoCyc: EG10939].

Uniprot Description

Function: Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation. Overexpression of some hybrid proteins has been thought to jam the protein secretion apparatus resulting in cell death; while this may be true it also results in FtsH-mediated degradation of SecY. Ref.9

Subunit structure: Part of the essential Sec protein translocation complex which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC/OxaA. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. The channel interacts with SecA via subunit SecY. SecE might contact ribosomal protein L23 and/or L29 when the translocation complex is docked with the ribosome. Ref.7 Ref.8

Subcellular location: Cell inner membrane; Multi-pass membrane protein Ref.10.

Post-translational modification: Degraded by FtsH when the SecYEG complex is jammed or upon treatment with antibiotics that block translation elongation such as chloramphenicol.

Disruption phenotype: Essential; deletion experiments lead to loss of the SecYEG tranlocation channel. Leads to loss of translocation of lipoproteins Lpp and BRP. Ref.9

Sequence similarities: Belongs to the SecE/SEC61-gamma family.