SDS-PAGE
Rhodobacter sphaeroides Modification methylase RsrI Recombinant Protein | rsrIM recombinant protein
Recombinant Rhodobacter sphaeroides Modification methylase RsrI
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Rhodobacter sphaeroides Modification methylase RsrI; Recombinant Rhodobacter sphaeroides Modification methylase RsrI; Adenine-specific methyltransferase RsrI; rsrIM recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-319aa; Full Length
Sequence
MANRSHHNAGHRAMNALRKSGQKHSSESQLGSSEIGTTRHVYDVCDCLDTLAKLPDDSVQLIICDPPYNIMLADWDDHMDYIGWAKRWLAEAERVLSPTGSIAIFGGLQYQGEAGSGDLISIISHMRQNSKMLLANLIIWNYPNGMSAQRFFANRHEEIAWFAKTKKYFFDLDAVREPYDEETKAAYMKDKRLNPESVEKGRNPTNVWRMSRLNGNSLERVGHPTQKPAAVIERLVRALSHPGSTVLDFFAGSGVTARVAIQEGRNSICTDAAPVFKEYYQKQLTFLQDDGLIDKARSYEIVEGAANFGAALQRGDVAS
Sequence Length
319
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
SDS-PAGE
SDS-PAGE
Related Product Information for rsrIM recombinant protein
This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease.
References
Purification, cloning and sequence analysis of RsrI DNA methyltransferase
lack of homology between two enzymes, RsrI and EcoRI, that methylate the same nucleotide in identical recognition sequences.Kaszubska W., Aiken C., O'Connor D., Gumport R.I., Stephenson F.H., Greene P.J.Nucleic Acids Res. 17:10403-10425(1989)
Nucleotide sequence of the gene encoding the RsrI methyltransferase.Stephenson F.H., Greene P.J.Nucleic Acids Res. 17:10503-10503(1989)
Structure of RsrI methyltransferase, a member of the N6-adenine beta class of DNA methyltransferases.Scavetta R.D., Thomas C.B., Walsh M.A., Szegedi S., Joachimiak A., Gumport R.I., Churchill M.E.Nucleic Acids Res. 28:3950-3961(2000)
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
51.7 kDa
NCBI Official Full Name
Modification methylase RsrI
UniProt Protein Name
Modification methylase RsrI
Protein Family
UniProt Gene Name
rsrIM
UniProt Synonym Gene Names
M.RsrI
UniProt Entry Name
MTR1_RHOSH
Uniprot Description
This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-? on both strands, and protects the DNA from cleavage by the RsrI endonuclease.
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Product Notes
The rsrIM rsrim (Catalog #AAA1187431) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-319aa; Full Length. The amino acid sequence is listed below: MANRSHHNAG HRAMNALRKS GQKHSSESQL GSSEIGTTRH VYDVCDCLDT LAKLPDDSVQ LIICDPPYNI MLADWDDHMD YIGWAKRWLA EAERVLSPTG SIAIFGGLQY QGEAGSGDLI SIISHMRQNS KMLLANLIIW NYPNGMSAQR FFANRHEEIA WFAKTKKYFF DLDAVREPYD EETKAAYMKD KRLNPESVEK GRNPTNVWRM SRLNGNSLER VGHPTQKPAA VIERLVRALS HPGSTVLDFF AGSGVTARVA IQEGRNSICT DAAPVFKEYY QKQLTFLQDD GLIDKARSYE IVEGAANFGA ALQRGDVAS. It is sometimes possible for the material contained within the vial of "Rhodobacter sphaeroides Modification methylase RsrI, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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