Endonuclease III (nth) Recombinant Protein | nth recombinant protein

Recombinant Escherichia coli Endonuclease III (nth)

Cat. Num. AAA1174154

• Gene Names: nth; ECK1629; JW1625
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Endonuclease III (nth); Recombinant Escherichia coli Endonuclease III (nth); nth recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-211, full length protein
• Sequence: MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI
• Sequence Length: 211

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16129591
• NCBI GeneID: 947122
• NCBI Accession #: NP_416150.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0AB83; P20625
• Molecular Weight: 23,562 Da
• NCBI Official Full Name: DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
• NCBI Official Symbol: nth
• NCBI Official Synonym Symbols: ECK1629; JW1625
• NCBI Protein Information: DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
• UniProt Protein Name: Endonuclease III
• Protein Family: Endonuclease
• UniProt Gene Name: nth

NCBI Description

Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].

Uniprot Description

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.