Uncharacterized protein YeaZ (yeaZ) Recombinant Protein | yeaZ recombinant protein

Recombinant Escherichia coli Uncharacterized protein YeaZ (yeaZ)

Cat. Num. AAA1146911

• Gene Names: tsaB; ECK1805; JW1796; rpf; yeaZ
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Uncharacterized protein YeaZ (yeaZ); Recombinant Escherichia coli Uncharacterized protein YeaZ (yeaZ); yeaZ recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-231, full length protein
• Sequence: MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQDILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGAWRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEWVTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYLRNNVAWKKLPGKE
• Sequence Length: 231

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16129761
• NCBI GeneID: 946304
• NCBI Accession #: NP_416321.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P76256; O08476; O08477
• Molecular Weight: 25,181 Da
• NCBI Official Full Name: tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD
• NCBI Official Symbol: tsaB
• NCBI Official Synonym Symbols: ECK1805; JW1796; rpf; yeaZ
• NCBI Protein Information: tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD
• UniProt Protein Name: tRNA threonylcarbamoyladenosine biosynthesis protein TsaB
• UniProt Gene Name: tsaB
• UniProt Synonym Gene Names: yeaZ

NCBI Description

AFMB Structural Genomics target Number 123 (http://afmb.cnrs-mrs.fr/article171.html). [More information is available at EcoGene: EG13512]. The preliminary analysis of a crystal structure of the predicted protease YeaZ has been published . [More information is available at EcoCyc: G6991].

Uniprot Description

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaE. TsaB seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. In fact, can act as a protease that specifically degrades TsaD in vitro; therefore TsaB may post-translationally regulate cellular pools of TsaD via proteolytic degradation. Does not show sialoglycoprotease activity against glycophorin A.