DNA-binding protein fis (fis) Recombinant Protein | fis recombinant protein

Recombinant Escherichia coli DNA-binding protein fis (fis)

Cat. Num. AAA1076730

• Gene Names: fis; ECK3248; JW3229; nbp
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-binding protein fis (fis); Recombinant Escherichia coli DNA-binding protein fis (fis); fis recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-98, full length protein
• Sequence: MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN
• Sequence Length: 98

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16131149
• NCBI GeneID: 947697
• NCBI Accession #: NP_417727.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A6R3; P11028; P37404; Q2M8V4
• Molecular Weight: 11,240 Da
• NCBI Official Full Name: global DNA-binding transcriptional dual regulator
• NCBI Official Symbol: fis
• NCBI Official Synonym Symbols: ECK3248; JW3229; nbp
• NCBI Protein Information: global DNA-binding transcriptional dual regulator
• UniProt Protein Name: DNA-binding protein Fis
• Protein Family: DNA-binding protein
• UniProt Gene Name: fis

NCBI Description

fis transcription is supercoiling-dependent. [More information is available at EcoGene: EG10317]. Fis, "factor for inversion stimulation", is a small DNA-binding and bending protein whose main role appears to be the organization and maintenance of nucleoid structure through direct DNA binding and by modulating gyrase and topoisomerase I production , as well as regulation of other proteins that modulate the nucleoid structure, such as CRP, HNS, and HU. [More information is available at EcoCyc: EG10317].

Uniprot Description

Activates ribosomal RNA transcription, as well other genes. Plays a direct role in upstream activation of rRNA promoters. Binds to a recombinational enhancer sequence that is required to stimulate hin-mediated DNA inversion. Prevents initiation of DNA replication from oriC. Binds to hundreds of transcriptionally active and inactive AT-rich sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome (PubMed:16963779).