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Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) Recombinant Protein | NCS6 recombinant protein

Recombinant Arabidopsis thaliana Cytoplasmic tRNA 2-thiolation protein 1 (NCS6)

Gene Names
ROL5; F4I1.8; repressor of lrx1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Cytoplasmic tRNA 2-thiolation protein 1 (NCS6); Recombinant Arabidopsis thaliana Cytoplasmic tRNA 2-thiolation protein 1 (NCS6); NCS6 recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-355, Full length protein
Sequence
MEAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGERVAIGASGGKDSTVLAYVLSELNRRHNYGLDLFLLSIDEGITGYRDDSLETVKRNEVQYGLPLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQALDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKYTYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRIATTTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPGSVAKSIESKQCGSLDF
Sequence Length
355
Species
Arabidopsis thaliana (Mouse-ear cress)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,279 Da
NCBI Official Full Name
repressor of lrx1
NCBI Official Symbol
ROL5
NCBI Official Synonym Symbols
F4I1.8; repressor of lrx1
NCBI Protein Information
repressor of lrx1
UniProt Protein Name
Cytoplasmic tRNA 2-thiolation protein 1
UniProt Gene Name
NCS6

NCBI Description

Encodes ROL5, a repressor of lrx1 mutants that develop aberrant root hairs. ROL5 is a homolog of yeast Ncs6p that affects TOR signaling. The target of rapamycin (TOR) pathway is a major regulator of cell growth in eukaryotes, and inhibition of this pathway by rapamycin reduces cell growth. ROL5 might function as a mitochondrial component of the TOR pathway that influences the plant's response to ROS (reactive oxygen species).

Uniprot Description

Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.

Research Articles on NCS6

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Product Notes

The NCS6 ncs6 (Catalog #AAA1042794) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-355, Full length protein. The amino acid sequence is listed below: MEAKNKKAVA SRLCCLCNLR RPVLKRPKTL QQICRECFYE VFEEEIHQVI VQNRLFKSGE RVAIGASGGK DSTVLAYVLS ELNRRHNYGL DLFLLSIDEG ITGYRDDSLE TVKRNEVQYG LPLKIVSYKD LYGWTMDEIV KMIGLKNNCT FCGVFRRQAL DRGAALLKVE KLVTGHNADD IAETVLLNIL RGDIARLSRC TSITTGEDGP IPRCKPFKYT YEKEIVMYAY FKKLDYFSTE CIYSPNAYRG FAREFIKDLE RIRPRAILDI IKSGEDFRIA TTTKMPEQGT CERCGYISSQ KWCKACVLLE GLNRGLPKMG IGRPRGVNGD HNKETKKPGS VAKSIESKQC GSLDF. It is sometimes possible for the material contained within the vial of "Cytoplasmic tRNA 2-thiolation protein 1 (NCS6), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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