Sequence
11-Beta-Hydroxysteroid Dehydrogenase Type 1 Recombinant Protein | HSD11b1 recombinant protein
Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)
Gene Names
HSD11B1; HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
11-Beta-Hydroxysteroid Dehydrogenase Type 1; Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1); 11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C; Member 1; HSD11b1 recombinant protein
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 50ug/mL (varies by lot)
Applicable Applications for HSD11b1 recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Residues
Ala2~Asn292
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
8.6
Usage
Reconstitute in ddH2O to a concentration of 0-0.1 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C.
Aliquot and store at -80°C.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C.
Aliquot and store at -80°C.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-PAGE
SDS-PAGE
Product Categories/Family for HSD11b1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 36.0kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
11-beta hydroxysteroid dehydrogenase type 1
NCBI Official Synonym Full Names
hydroxysteroid 11-beta dehydrogenase 1
NCBI Official Symbol
HSD11B1
NCBI Official Synonym Symbols
HDL; 11-DH; HSD11; HSD11B; HSD11L; CORTRD2; SDR26C1; 11-beta-HSD1
NCBI Protein Information
corticosteroid 11-beta-dehydrogenase isozyme 1
Protein Family
NCBI Description
The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Mutations in this gene and H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase)) are the cause of cortisone reductase deficiency. Alternate splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2011]
Research Articles on HSD11b1
Similar Products
Product Notes
The HSD11b1 (Catalog #AAA2122938) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's 11-Beta-Hydroxysteroid Dehydrogenase Type 1 can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the HSD11b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "11-Beta-Hydroxysteroid Dehydrogenase Type 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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