ZWINT recombinant protein

ZWINT, 1-230aa, Human, His tag, E Coli

Cat. Num. AAA205745

• Gene Names: ZWINT; SIP30; ZWINT1; KNTC2AP; HZwint-1
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: ZWINT; 1-230aa; Human; His tag; E Coli; ZW10 interactor isoform b; HZwint-1; KNTC2AP; ZWINT1; ZWINT recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP
• Sequence Length: 230

• Applicable Applications for ZWINT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ZWINT recombinant protein

ZW10 interactor isoform b, also known as ZWINT, is clearly involved in kinetochore function. Localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase, ZWINT interacts with ZW10 (Zeste White 10) and functions to regulate the association between ZW10 and kinetochores. Defects in the gene encoding ZWINT are associated with the pathogenesis of Roberts's syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation. Recombinant human ZWINT protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ZWINT recombinant protein

Cell Cycle

References

Musio A., et al. (2004) Gene. 331: 33-40.; Lin Y T., et al. (2006) Oncogene. 25: 6901-6914.;

NCBI and Uniprot Product Information

• NCBI GI #: 53729320
• NCBI GeneID: 11130
• NCBI Accession #: NP_001005413
• NCBI GenBank Nucleotide #: NM_001005413.1
• UniProt Accession #: O95229; Q0D2I3; Q9BWD0; A6NNV6
• Molecular Weight: 27.9 kDa (253aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: ZW10 interactor isoform b
• NCBI Official Synonym Full Names: ZW10 interacting kinetochore protein
• NCBI Official Symbol: ZWINT
• NCBI Official Synonym Symbols: SIP30; ZWINT1; KNTC2AP; HZwint-1
• NCBI Protein Information: ZW10 interactor
• UniProt Protein Name: ZW10 interactor
• Protein Family: ZW10 interactor
• UniProt Gene Name: ZWINT
• UniProt Synonym Gene Names: Zwint-1

NCBI Description

This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.