Mouse Zinc finger MYND domain-containing protein 11 ELISA Kit | ZMYND11 elisa kit

Mouse Zinc finger MYND domain-containing protein 11 (ZMYND11) ELISA Kit

Cat. Num. AAA282292

• Gene Names: Zmynd11; BS69; 2210402G22Rik
• Reactivity: Mouse
• Synonyms: Zinc finger MYND domain-containing protein 11; Mouse Zinc finger MYND domain-containing protein 11 (ZMYND11) ELISA Kit; ZMYND11 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZMYND11. No significant cross-reactivity or interference between Mouse ZMYND11 and analogues was observed.
• Sequence Length: 602

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZMYND11 elisa kit

ZMYND11 was first identified by its ability to bind the adenovirus E1A protein. The protein localizes to the nucleus. It functions as a transcriptional repressor, and expression of E1A inhibits this repression. Alternatively spliced transcript variants encoding different isoforms have been identified.The deduced 562-amino acid protein had an apparent molecular mass of about 69 kD following in vitro transcription and translation. BS69 contains 2 N-terminal zinc finger motifs, followed by a nuclear localization signal and a C-terminal E1A-binding domain. Northern blot analysis detected a 4.7-kb transcript in all mouse tissues examined, with highest expression in kidney.The deduced BRAM1 protein contains the C-terminal 186 amino acids of BS69 preceded by 12 amino acids unique to BRAM1.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMYND11 in samples. An antibody specific for ZMYND11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMYND11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMYND11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMYND11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 312433970
• NCBI GeneID: 66505
• NCBI Accession #: NP_001186070.1
• NCBI GenBank Nucleotide #: NM_001199141.2
• UniProt Accession #: Q8R5C8; Q8C155; G5E8Q2
• Molecular Weight: 70,838 Da
• NCBI Official Full Name: zinc finger MYND domain-containing protein 11 isoform gamma
• NCBI Official Synonym Full Names: zinc finger, MYND domain containing 11
• NCBI Official Symbol: Zmynd11
• NCBI Official Synonym Symbols: BS69; 2210402G22Rik
• NCBI Protein Information: zinc finger MYND domain-containing protein 11
• UniProt Protein Name: Zinc finger MYND domain-containing protein 11
• Protein Family: Zinc finger MYND domain-containing protein
• UniProt Gene Name: Zmynd11

Uniprot Description

ZMYND11: Corepressor of transcription (Probable).

Protein type: DNA-binding; Transcription regulation; Tumor suppressor

Chromosomal Location of Human Ortholog: 13|13 A1

Cellular Component: chromosome; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; double-stranded DNA binding; histone binding; metal ion binding; methylated histone residue binding; transcription corepressor activity; zinc ion binding

Biological Process: covalent chromatin modification; defense response to virus; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of JNK cascade; negative regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent