Mouse Zinc finger protein 36, C3H1 type-like 2 ELISA Kit | ZFP36L2 elisa kit

Mouse Zinc finger protein 36, C3H1 type-like 2 (ZFP36L2) ELISA Kit

Cat. Num. AAA282366

• Gene Names: Zfp36l2; Brf2; ERF2; Tis11d
• Reactivity: Mouse
• Synonyms: Zinc finger protein 36; C3H1 type-like 2; Mouse Zinc finger protein 36; C3H1 type-like 2 (ZFP36L2) ELISA Kit; ZFP36L2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZFP36L2. No significant cross-reactivity or interference between Mouse ZFP36L2 and analogues was observed.
• Sequence Length: 494

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZFP36L2 elisa kit

ZF36L2 gene contains 2 exons. Except for the 3-prime UTR, the entire gene is GC rich. The promoter region contains a GC box, a CAT box, and a CRE element. The 3-prime UTR contains 5 copies of the ATTTA sequence, a characteristic of unstable mRNAs.The deduced 493-amino acid protein has a calculated molecular mass of 51 kD. It contains a zinc finger domain, polyglutamine and polyproline tracts that may act as transcriptional activators, a polyalanine tract, and 3 putative phosphorylation sites. Clusters of serine, proline, alanine, and glycine account for nearly half of the ERF2 sequence. Human ERF2 contains 97 additional amino acids at its C terminus relative to mouse Tis11d, but it shares 90% amino acid identity with mouse Tis11d prior to the C-terminal divergence.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFP36L2 in samples. An antibody specific for ZFP36L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFP36L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFP36L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFP36L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 49249965
• NCBI GeneID: 12193
• NCBI Accession #: NP_001001806.1
• NCBI GenBank Nucleotide #: NM_001001806.2
• UniProt Accession #: P23949; Q3TCE3; Q3TU59; B9EIF6
• Molecular Weight: 50,070 Da
• NCBI Official Full Name: mRNA decay activator protein ZFP36L2
• NCBI Official Synonym Full Names: zinc finger protein 36, C3H type-like 2
• NCBI Official Symbol: Zfp36l2
• NCBI Official Synonym Symbols: Brf2; ERF2; Tis11d
• NCBI Protein Information: mRNA decay activator protein ZFP36L2
• UniProt Protein Name: mRNA decay activator protein ZFP36L2
• Protein Family: mRNA decay activator protein
• UniProt Gene Name: Zfp36l2
• UniProt Synonym Gene Names: ZFP36-like 2

Uniprot Description

ZFP36L2: Probable regulatory protein involved in regulating the response to growth factors. RNA-binding protein that binds to 5'UUAUUUAUUU-3' core sequence. Binds to the class II AU-rich element (ARE) in the 3'-UTR of target mRNAs and promotes their deadenylation and degradation.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 17|17 E4

Cellular Component: cytoplasm; cytosol; intracellular ribonucleoprotein complex; nucleus

Molecular Function: AU-rich element binding; DNA binding; metal ion binding; RNA binding

Biological Process: hemopoiesis; MAPK cascade; mRNA catabolic process; mRNA catabolic process, deadenylation-dependent decay; multicellular organism development; negative regulation of fat cell differentiation; regulation of B cell differentiation; regulation of mRNA stability; response to wounding; somatic stem cell division; somatic stem cell maintenance; T cell differentiation in thymus