Mouse Zinc finger homeobox protein 3 ELISA Kit | ZFHX3 elisa kit

Mouse Zinc finger homeobox protein 3 (ZFHX3) ELISA Kit

Cat. Num. AAA280797

• Gene Names: Zfhx3; Sci; WBP9; Atbf1; mKIAA4228; A230102L03Rik
• Reactivity: Mouse
• Synonyms: Zinc finger homeobox protein 3; Mouse Zinc finger homeobox protein 3 (ZFHX3) ELISA Kit; ZFHX3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse ZFHX3. No significant cross-reactivity or interference between Mouse ZFHX3 and analogues was observed.
• Sequence Length: 3703

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZFHX3 elisa kit

Tissue-specific expression of the human alpha-fetoprotein (AFP) gene is strongly stimulated by an enhancer present 3.3 to 4.9 kb upstream of the transcription initiation site. To determine the nuclear factor in hepatoma cell lines that interacts with the human AFP enhancer AT motif, Morinaga et al. (1991) screened a hepatoma cDNA expression library with an AFP enhancer fragment that bore the AT motif. They succeeded in isolating a cDNA that can code for an AT motif-binding factor, which they termed ATBF1. This was the largest DNA-binding protein identified to that time and the first protein shown to contain multiple homeodomains and multiple zinc finger motifs. The protein had a predicted mass of 306 kD and contained 4 homeodomains and 17 zinc finger motifs.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZFHX3 in samples. An antibody specific for ZFHX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZFHX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZFHX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZFHX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 18202592
• NCBI GeneID: 11906
• UniProt Accession #: Q61329
• Molecular Weight: 406,570 Da
• NCBI Official Full Name: Zinc finger homeobox protein 3
• NCBI Official Synonym Full Names: zinc finger homeobox 3
• NCBI Official Symbol: Zfhx3
• NCBI Official Synonym Symbols: Sci; WBP9; Atbf1; mKIAA4228; A230102L03Rik
• NCBI Protein Information: zinc finger homeobox protein 3
• UniProt Protein Name: Zinc finger homeobox protein 3
• Protein Family: Zinc finger homeobox protein
• UniProt Gene Name: Zfhx3
• UniProt Synonym Gene Names: Atbf1; ZFH-3

Uniprot Description

ATBF1: Transcriptional repressor. It inhibits the enhancer element of the AFP gene by binding to its AT-rich core sequence. Regulator of myoblasts differentiation through the binding to the AT-rich sequence of MYF6 promoter and promoter repression. Down- regulates the MUC5AC promoter in gastric cancer. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Cell development/differentiation; DNA-binding; Mitochondrial; Transcription factor

Chromosomal Location of Human Ortholog: 8|8 D3

Cellular Component: cytoplasm; mitochondrion; nuclear body; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; enzyme binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding; zinc ion binding

Biological Process: circadian regulation of gene expression; muscle organ development; negative regulation of myoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; transcription, DNA-dependent