Typical Testing Data/Standard Curve (for reference only)
Rat Adipocytokine zinc alpha 2 glycoprotein ELISA Kit | ZAG elisa kit
Rat Adipocytokine zinc alpha 2 glycoprotein ELISA Kit
Reactivity
Rat
Synonyms
Adipocytokine zinc alpha 2 glycoprotein; Rat Adipocytokine zinc alpha 2 glycoprotein ELISA Kit; ZAG elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5-100ng/mL
Sensitivity
1.0ng/ml
Intended Uses
This ZAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ZAG. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ZAG elisa kit
Principle of the assay: ZAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZAG antibody and an ZAG-HRP conjugate. The assay sample and buffer are incubated together with ZAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZAG concentration since ZAG from samples and ZAG-HRP conjugate compete for the anti-ZAG antibody binding site. Since the number of sites is limited, as more sites are occupied by ZAG from the sample, fewer sites are left to bind ZAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZAG concentration in each sample is interpolated from this standard curve.
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Product Notes
The Rat ZAG (Catalog #AAA755131) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755131 ELISA Kit recognizes Rat ZAG. It is sometimes possible for the material contained within the vial of "Adipocytokine zinc alpha 2 glycoprotein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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