YY1 recombinant protein

Recombinant YY1 protein

Cat. Num. AAA389176

• Gene Names: YY1; DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• Synonyms: YY1; Recombinant YY1 protein; DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1; YY1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant YY1 protein is supplied at a concentration of 0.3ug/ul in 25mM HEPES pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This protein is suitable for use in protein-protein interaction, in vitro transcription assay, binding assay. Binding Assay Condition: 1uM oligo ds DNA (DNA sequence 5'-GCGCCGCCATCTTCAT-3') was incubated with different concentrations of YY1 protein in binding system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant YY1 protein gel 7.5% SDS-PAGE Coomassie staining MW: 46 kDa Purity: >85% )

Testing Data

(HTRF assay for YY1 activity 1 uM oligo dsDNA was incubated with different concentrations of YY1 protein in for 1 hr, then DYKDDDDK antibody and SA-XL665 mixture was added to each reaction system and incubated for 30 min. HTRF assay was used for detection. )

Related Product Information for YY1 recombinant protein

Short Description: Recombinant human YY1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_003394.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 46 kDa. It is suitable for use in protein-protein interaction studies, in vitro transcription assays and binding assays.

Background: YY1 (YY1 Transcription Factor, also known as INO80S) is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. YY1 transcription factor,repressor/activator (Yin Yang) protein, is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. It binds to the consensus sequence 5'-CCGCCATNTT-3'; some genes have been shown to contain a longer binding motif allowing enhanced binding; the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. YY1 is also involved in DNA repair. In vitro, it binds to DNA recombination intermediate structures (Holliday junctions).

Product Categories/Family for YY1 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 459683878
• NCBI GeneID: 7528
• NCBI Accession #: NP_003394.1
• NCBI GenBank Nucleotide #: NM_003403
• Molecular Weight: 44,713 Da
• NCBI Official Full Name: Homo sapiens YY1 transcription factor (YY1), mRNA
• NCBI Official Synonym Full Names: YY1 transcription factor
• NCBI Official Symbol: YY1
• NCBI Official Synonym Symbols: DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1
• NCBI Protein Information: transcriptional repressor protein YY1; INO80 complex subunit S; YY-1; Yin and Yang 1 protein; delta transcription factor
• Protein Family: YY1-associated factor

NCBI Description

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq, Jul 2008]