Human tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide ELISA Kit | YWHAQ elisa kit

Human 14-3-3 protein theta, YWHAQ ELISA Kit

Cat. Num. AAA927389

• Gene Names: YWHAQ; 1C5; HS1; 14-3-3
• Reactivity: Human
• Synonyms: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; theta polypeptide; Human 14-3-3 protein theta; YWHAQ ELISA Kit; Human 14-3-3 protein theta (YWHAQ) ELISA kit; 14-3-3; 1C5; HS1; 14-3-3 protein T-cell; 14-3-3 protein tau; 14-3-3 protein theta; OTTHUMP00000165887; protein tau; tyrosine 3/tryptophan 5 -monooxygenase activation protein; YWHAQ elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human YWHAQ. No significant cross-reactivity or interference between Human YWHAQ and analogues was observed.
• Sequence Length: 245

• Samples: Serum, plasma, tissue homogenates and cerebrospinal fluid (CSF)
• Assay Type: Sandwich
• Detection Range: 0.47 ng/ml-30 ng/ml
• Sensitivity: 0.11 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for YWHAQ elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YWHAQ bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 5803227
• NCBI GeneID: 10971
• NCBI Accession #: NP_006817.1
• NCBI GenBank Nucleotide #: NM_006826.3
• UniProt Accession #: P27348; Q567U5; Q5TZU8; Q9UP48; D6W4Z5
• Molecular Weight: 27,764 Da
• NCBI Official Full Name: 14-3-3 protein theta
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta
• NCBI Official Symbol: YWHAQ
• NCBI Official Synonym Symbols: 1C5; HS1; 14-3-3
• NCBI Protein Information: 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• UniProt Protein Name: 14-3-3 protein theta
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAQ
• UniProt Entry Name: 1433T_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 theta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Associates with c-BCR and BCR-Abl.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p25.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; protein N-terminus binding

Biological Process: substantia nigra development; apoptosis; small GTPase mediated signal transduction; negative regulation of transcription, DNA-dependent; protein targeting