14-3-3gamma Recombinant Protein | YWHAG recombinant protein

14-3-3gamma (human), (recombinant) (GST-tag)

Cat. Num. AAA566613

• Gene Names: YWHAG; 14-3-3GAMMA
• Purity: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Synonyms: 14-3-3gamma; 14-3-3gamma (human); (recombinant) (GST-tag); YWHAG recombinant protein

• Host: E Coli
• Purity/Purification: >=90% (SDS-PAGE); Purified by multi-step chromatography
• Form/Format: Liquid. In 50mM TRIS/HCl, pH7.5, 150mM NaCl

• Source Note: Human 14-3-3gamma is fused at the N-terminus to a GST-tag
• Preparation and Storage: For long term storage, store at -80 degree C; Shipping: Shipped on Dry Ice

Related Product Information for YWHAG recombinant protein

14-3-3 proteins are dimeric phosphoserine/phosphothreonine binding proteins, which have been shown to be involved in the regulation of many crucial processes such as signal transduction, cell-cycle control, apoptosis, protein localization, phosphorylation-state, stability, transcription, metabolism, and malignant transformation. Potential 14-3-3 gamma protein-protein interactions are also differentially regulated by cytokine stimulation. One report showed that 14-3-3 gamma expression is induced in arterial trauma by cytokines, which suggests that this protein may play an important role in progression of vascular proliferative diseases.

Product Categories/Family for YWHAG recombinant protein

Cellular Analysis; DNA Regulation & Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 21464101
• NCBI GeneID: 7532
• NCBI Accession #: NP_036611.2
• NCBI GenBank Nucleotide #: NM_012479.3
• UniProt Accession #: P61981; O70457; P35214; Q6FH52; Q9UDP2; Q9UN99
• Molecular Weight: 28,303 Da
• NCBI Official Full Name: 14-3-3 protein gamma
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
• NCBI Official Symbol: YWHAG
• NCBI Official Synonym Symbols: 14-3-3GAMMA
• NCBI Protein Information: 14-3-3 protein gamma; KCIP-1; 14-3-3 gamma; protein kinase C inhibitor protein 1
• UniProt Protein Name: 14-3-3 protein gamma
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAG
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433G_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 gamma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Homodimer. Interacts with SAMSN1. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT. Highly expressed in brain, skeletal muscle, and heart. Belongs to the 14-3-3 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; cytosol

Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; insulin-like growth factor receptor binding; protein binding; protein kinase C binding; receptor tyrosine kinase binding

Biological Process: cellular response to insulin stimulus; apoptosis; regulation of neuron differentiation; organelle organization and biogenesis; regulation of signal transduction; regulation of synaptic plasticity; negative regulation of protein kinase activity; mitotic cell cycle; G2/M transition of mitotic cell cycle; protein targeting