Mouse anti-Human CENPQ Monoclonal Antibody | anti-CENPQ antibody

CENPQ (C6orf139, Centromere Protein Q, CENP-Q, FLJ10545)

Cat. Num. AAA642599

• Gene Names: CENPQ; CENP-Q; C6orf139
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CENPQ; Monoclonal Antibody; CENPQ (C6orf139; Centromere Protein Q; CENP-Q; FLJ10545); Anti -CENPQ (C6orf139; anti-CENPQ antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2B5-2D12
• Specificity: Recognizes human C6orf139.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSGKANASKKNAQQLKRNPKRKKDNEEVVLSENKVRNTVKKNKNHLKDLSSEGQTKHTNLKHGKTAASKRKTWQPLSKSTRDHLQTMMESVIMTILSNSIKEKEEIQYHLNFLKKRLLQQCETLKVPPKKMEDLTNVSSLLNMERARDKANEEGLALLQEEIDKMVETTELMTGNIQSLKNKIQILASEVEEEEERVKQMHQINSSGVLSLPELSQKTLKAPTLQKEILALIPNQNALLKDLDILHNSSQMKSMSTFIEEAYKKLDAS

• Applicable Applications for anti-CENPQ antibody: ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length recombinant corresponding to aa1-268 from human C6orf139 (AAH16279) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (55.59kD).)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to C6orf139 on HeLa cell . [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged C6orf139 is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-CENPQ antibody

CENPQ is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).

Product Categories/Family for anti-CENPQ antibody

Antibodies; Abs to Centromeres

NCBI and Uniprot Product Information

• NCBI GI #: 40068061
• NCBI GeneID: 55166
• NCBI Accession #: NP_060602.2
• NCBI GenBank Nucleotide #: NM_018132.3
• UniProt Accession #: Q7L2Z9; Q6IN61; Q9NVS5; A8KAF1
• Molecular Weight: 30,595 Da
• NCBI Official Full Name: centromere protein Q
• NCBI Official Synonym Full Names: centromere protein Q
• NCBI Official Symbol: CENPQ
• NCBI Official Synonym Symbols: CENP-Q; C6orf139
• NCBI Protein Information: centromere protein Q
• UniProt Protein Name: Centromere protein Q
• Protein Family: Centromere protein
• UniProt Gene Name: CENPQ
• UniProt Synonym Gene Names: C6orf139; CENP-Q
• UniProt Entry Name: CENPQ_HUMAN

NCBI Description

CENPQ is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]

Uniprot Description

CENPQ: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex.

Chromosomal Location of Human Ortholog: 6p12.3

Cellular Component: nucleoplasm; cytosol; chromosome, pericentric region; actin cytoskeleton

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle