14-3-3 epsilon Blocking Peptide | YWHAE blocking peptide

14-3-3 epsilon Blocking Peptide

Cat. Num. AAA9623665

• Gene Names: YWHAE; MDS; MDCR; KCIP-1; 14-3-3E
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: 14-3-3 epsilon; 14-3-3 epsilon Blocking Peptide; 14 3 3 E; 14 3 3 epsilon; 14 3 3E; 14-3-3 protein epsilon; 14-3-3E; 1433E_HUMAN; Epididymis luminal protein 2; FLJ45465; FLJ53559; HEL2; KCIP 1; KCIP1; MDCR; MDS; Mitochondrial import stimulation factor L subunit; Protein kinase C inhibitor protein1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; epsilon; epsilon polypeptide; Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide; YWHAE; YWHAE blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for YWHAE blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for YWHAE blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 5803225
• NCBI GeneID: 7531
• NCBI Accession #: NP_006752.1
• NCBI GenBank Nucleotide #: NM_006761.4
• UniProt Accession #: P62258; P29360; P42655; Q4VJB6; Q53XZ5; Q63631; Q7M4R4; B3KY71; D3DTH5
• Molecular Weight: 29,174 Da
• NCBI Official Full Name: 14-3-3 protein epsilon
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• NCBI Official Symbol: YWHAE
• NCBI Official Synonym Symbols: MDS; MDCR; KCIP-1; 14-3-3E
• NCBI Protein Information: 14-3-3 protein epsilon; 14-3-3 epsilon; protein kinase C inhibitor protein-1; mitochondrial import stimulation factor L subunit; tyrosine 3/tryptophan 5 -monooxygenase activation protein, epsilon polypeptide
• UniProt Protein Name: 14-3-3 protein epsilon
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAE
• UniProt Synonym Gene Names: 14-3-3E
• UniProt Entry Name: 1433E_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

14-3-3 epsilon: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: kinesin complex; cytoplasmic vesicle membrane; focal adhesion; mitochondrion; membrane; axon; melanosome; cytosol

Molecular Function: protein domain specific binding; protein binding; enzyme binding; potassium channel regulator activity; protein heterodimerization activity; histone deacetylase binding; phosphoprotein binding; phosphoserine binding

Biological Process: substantia nigra development; nerve growth factor receptor signaling pathway; viral reproduction; apoptosis; organelle organization and biogenesis; hippocampus development; neuron migration; mitotic cell cycle; cerebral cortex development; regulation of caspase activity; G2/M transition of mitotic cell cycle; regulation of the rate of heart contraction by hormone; protein targeting

Disease: Miller-dieker Lissencephaly Syndrome