Mouse YTH domain family protein 1 ELISA Kit | YTHDF1 elisa kit

Mouse YTH domain family protein 1 (YTHDF1) ELISA Kit

Cat. Num. AAA280374

• Gene Names: Ythdf1; 8030473O16; 2210410K23Rik
• Reactivity: Mouse
• Synonyms: YTH domain family protein 1; Mouse YTH domain family protein 1 (YTHDF1) ELISA Kit; YTHDF1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse YTHDF1. No significant cross-reactivity or interference between Mouse YTHDF1 and analogues was observed.
• Sequence Length: 561

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for YTHDF1 elisa kit

YTHDF1, Contains 1 YTH domain. The YTH (YT521-B homology) domain was identified by sequence comparison and is found in 174 different proteins expressed in eukaryotes. It is characterized by 14 invariant residues within an alpha-helix/beta-sheet structure. YTH domain is a novel RNA binding domain that binds to a short, degenerated, single-stranded RNA sequence motif. The presence of the binding motif in alternative exons is necessary for YT521-B to directly influence splice site selection in vivo. Array analyses demonstrate that YT521-B predominantly regulates vertebrate-specific exons. An NMR titration experiment identified the binding surface for single-stranded RNA on the YTH domain. Structural analyses indicate that the YTH domain is related to the pseudouridine synthase and archaeosine transglycosylase (PUA) domain.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YTHDF1 in samples. An antibody specific for YTHDF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYTHDF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YTHDF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YTHDF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 30424609
• NCBI GeneID: 228994
• NCBI Accession #: NP_776122.1
• NCBI GenBank Nucleotide #: NM_173761.3
• UniProt Accession #: P59326; Q3T9E2
• Molecular Weight: 60,879 Da
• NCBI Official Full Name: YTH domain-containing family protein 1
• NCBI Official Synonym Full Names: YTH domain family 1
• NCBI Official Symbol: Ythdf1
• NCBI Official Synonym Symbols: 8030473O16; 2210410K23Rik
• NCBI Protein Information: YTH domain-containing family protein 1
• UniProt Protein Name: YTH domain-containing family protein 1
• Protein Family: YTH domain-containing family protein
• UniProt Gene Name: Ythdf1
• UniProt Synonym Gene Names: DACA-1 homolog

Uniprot Description

YTHDF1: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 2|2 H4

Cellular Component: cytoplasm

Molecular Function: ribosome binding; RNA binding

Biological Process: positive regulation of translation; positive regulation of translational initiation