YTH domain-containing protein 1 (Ythdc1) Recombinant Protein | Ythdc1 recombinant protein

Recombinant Rat YTH domain-containing protein 1 (Ythdc1)

Cat. Num. AAA1397861

• Gene Names: Ythdc1; Yt521
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: YTH domain-containing protein 1 (Ythdc1); Recombinant Rat YTH domain-containing protein 1 (Ythdc1); Ythdc1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-738, full length protein
• Sequence: MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSERMESIDTKRQKPSIHSRQLISKPLSSSVSNNKRIVSTKGKSVTEYKNEEYQRSERNKRLDADRKIRLSSSSSREPYKSQPEKPCLRKRDSERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEGNSEEYGSDHETGSSASSEQGNNTENEEEGGEEDVEEDEEVDEDGDDDEEVDEDAEEEEDEEEDEEEEDEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSFTDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYAGSEKKHEKLSSSVRAVRKDQTSKLKSVLQDARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQLIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDSFTNLIPNRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
• Sequence Length: 738

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 19173798
• NCBI GeneID: 170956
• NCBI Accession #: NP_596914.1
• NCBI GenBank Nucleotide #: NM_133423.1
• UniProt Accession #: Q9QY02; O54729; G3V690
• Molecular Weight: 83,006 Da
• NCBI Official Full Name: YTH domain-containing protein 1
• NCBI Official Synonym Full Names: YTH domain containing 1
• NCBI Official Symbol: Ythdc1
• NCBI Official Synonym Symbols: Yt521
• NCBI Protein Information: YTH domain-containing protein 1
• UniProt Protein Name: YTH domain-containing protein 1
• Protein Family: YTH domain-containing protein
• UniProt Gene Name: Ythdc1

NCBI Description

binds nuclear transcriptosomal complex scaffold attachment factor B (SAF-B) and Sam68; may play a role in splice site selection and alternative splicing [RGD, Feb 2006]

Uniprot Description

Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and acts as a regulator of alternative splicing (PubMed:25389274). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:25389274). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (). May also regulate alternative splice site selection (PubMed:10564280, PubMed:9473574). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs ().