Alpha-glucosidase yihQ (yihQ) Recombinant Protein | yihQ recombinant protein

Recombinant Escherichia coli Alpha-glucosidase yihQ (yihQ)

Cat. Num. AAA1112727

• Gene Names: yihQ; ECK3871; JW3849; squQ
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Alpha-glucosidase yihQ (yihQ); Recombinant Escherichia coli Alpha-glucosidase yihQ (yihQ); yihQ recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-678, full length protein
• Sequence: MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSGIADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGRLLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQTYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALWEDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMRNAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYINPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKKNMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEILFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGYTTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFTTLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRSDWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI
• Sequence Length: 678

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16131718
• NCBI GeneID: 948376
• NCBI Accession #: NP_418314.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P32138; P76775; Q2M8H5
• Molecular Weight: 77,275 Da
• NCBI Official Full Name: putative sulpholipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase
• NCBI Official Symbol: yihQ
• NCBI Official Synonym Symbols: ECK3871; JW3849; squQ
• NCBI Protein Information: putative sulpholipid alpha-glucosidase; alpha-glucosyl fluoride glucosidase
• UniProt Protein Name: Sulfoquinovosidase
• Protein Family: Sulfoquinovosidase
• UniProt Gene Name: yihQ
• UniProt Synonym Gene Names: SQase

NCBI Description

YihQ has hydrolysis activity against alpha-glucosyl fluoride. yihO-U may be a sugar catabolism cluster/operon. [More information is available at EcoGene: EG11843]. YihQ is a member of the glycosyl hydrolase 31 family and has -glycosidase activity. [More information is available at EcoCyc: EG11843].

Uniprot Description

Catalyzes the hydrolysis of sulfoquinovosyl diacylglycerides (SQDG) to sulfoquinovose (SQ), which is then degraded by E.coli through the SQ Embden-Meyerhof-Parnas (SQ-EMP) sulfoglycolysis pathway as a source of carbon and sulfur. Therefore, is likely involved in the utilization of the sulfoquinovose headgroup found in ubiquitous plant sulfolipids. Is also able to hydrolyze simple sulfoquinovosides such as 1-sulfoquinovosylglycerol (SQGro). Is a retaining glycoside hydrolase, since it forms the alpha anomer of SQ (PubMed:26878550). Also exhibits some alpha-glucosidase activity against alpha-glucosyl fluoride in vitro, although natural substrates, such as alpha-glucobioses are scarcely hydrolyzed (PubMed:15294295).