COL4A3BP recombinant protein

COL4A3BP Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8121664

• Gene Names: COL4A3BP; CERT; GPBP; CERTL; STARD11
• Purity: >90% as determined by SDS-PAGE
• Synonyms: COL4A3BP; COL4A3BP Protein; Human; Recombinant (His & GST Tag); Human COL4A3BP Protein (His & GST Tag); CERT Protein; CERTL Protein; GPBP Protein; STARD11 Protein; collagen; type IV; alpha 3 (Goodpasture antigen) binding protein; COL4A3BP recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM Nacl, pH7.4, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Phe598

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human COL4A3BP (Q9Y5P4-1)(Met1-Phe598) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for COL4A3BP recombinant protein

Background: COL4A3BP is a member of the StarD2 subfamily. It contains a pleckstrin homology domain at its amino terminus and a START domain towards the end of the molecule. COL4A3BP has a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. One isoform of COL4A3BP is also involved in ceramide intracellular transport. COL4A3BP specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. An autoimmune response directed at this antigen can cause goodpasture disease.

References

Rual JF, et al. (2005) Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437(7062):1173-8. Granero F, et al. (2005) A human-specific TNF-responsive promoter for Goodpasture antigen-binding protein. FEBS J. 272(20):5291-305. Longo I, et al. (2006) Autosomal recessive Alport syndrome: an in-depth clinical and molecular analysis of five families. Nephrol Dial Transplant. 21(3):665-71.

NCBI and Uniprot Product Information

• NCBI GI #: 5031717
• NCBI GeneID: 10087
• NCBI Accession #: NP_005704.1
• NCBI GenBank Nucleotide #: NM_005713.2
• UniProt Accession #: Q9Y5P4; Q53YV1; Q53YV2; Q96Q85; Q96Q88; Q9H2S7; Q9H2S8; A8K7S2; B3KUB7
• Molecular Weight: 70,835 Da
• NCBI Official Full Name: collagen type IV alpha-3-binding protein isoform 1
• NCBI Official Synonym Full Names: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• NCBI Official Symbol: COL4A3BP
• NCBI Official Synonym Symbols: CERT; GPBP; CERTL; STARD11
• NCBI Protein Information: collagen type IV alpha-3-binding protein; hCERT; ceramide transporter; ceramide transfer protein; lipid-transfer protein CERTL; StAR-related lipid transfer (START) domain containing 11
• UniProt Protein Name: Collagen type IV alpha-3-binding protein
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: COL4A3BP
• UniProt Synonym Gene Names: CERT; STARD11; hCERT; GPBP; StARD11
• UniProt Entry Name: C43BP_HUMAN

NCBI Description

This gene encodes a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

COL4A3BP: a protein with a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. Implicated in human autoimmune diseases via its association with the Goodpasture antigen. Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. GPBP has been reported to have Ser/Thr protein kinase activity towards the Goodpasture (GP) antigen, but lacks a conventional serine/threonine kinase domain. Two human isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.9; Lipid-binding; Kinase, protein; Protein kinase, atypical; ATYPICAL group; COL4A3BP family

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: nucleoplasm; Golgi apparatus; endoplasmic reticulum membrane; mitochondrion; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; protein kinase activity

Biological Process: heart morphogenesis; endoplasmic reticulum organization and biogenesis; sphingolipid metabolic process; sphingolipid biosynthetic process; in utero embryonic development; cell morphogenesis; lipid homeostasis; signal transduction; protein amino acid phosphorylation; cell proliferation; muscle contraction; immune response; ceramide metabolic process

Disease: Mental Retardation, Autosomal Dominant 34