Putative diguanylate cyclase YeaJ (yeaJ) Recombinant Protein | yeaJ recombinant protein
Recombinant Escherichia coli Putative diguanylate cyclase YeaJ (yeaJ)
NCBI and Uniprot Product Information
NCBI Description
A yeaJ null mutation partially suppresses the motility defect of a yhjH mutation at 37C; a yegE null mutation is a better yhjH suppressor at 28C; full yhjH suppression requires yegE, yedQ and yeaJ null mutations when grown at 37C (Pesavento, 2008). [More information is available at EcoGene: EG13496].
Uniprot Description
Function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.
Catalytic activity: 2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate.
Cofactor: Binds 1 Mg2+ per monomer
By similarity.
Pathway: Purine metabolism; 3',5'-cyclic di-GMP biosynthesis.
Subunit structure: Homodimer
By similarity.
Subcellular location: Cell inner membrane; Multi-pass membrane protein
Potential.
Induction: Expressed during exponential and post-exponential growth, it is slightly more highly expressed at 37 than 28 degrees Celsius and is more highly induced on solid medium. Ref.5
Disruption phenotype: No visible phenotype. Disruption of this gene partially suppresses the reduced motility of a yhjH disruption. Full suppression i.e. wild-type motility, requires concomitant disruption of yeaJ, yegE and yedQ. Ref.4
Sequence similarities: Contains 1 GGDEF domain.
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Product Notes
The yeaJ yeaj (Catalog #AAA1077894) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-496. The amino acid sequence is listed below: MKLHHRMLRH FIAASVIVLT SSFLIFELVA SDRAMSAYLR YIVQKADSSF LYDKYQNQSI AAHVMRALAA EQSEVSPEQR RAICEAFESA NNTHGLNLTA HKYPGLRGTL QTASTDCDTI VEAAALLPAF DQAVEGNRHQ DDYGSGLGMA EEKFHYYLDL NDRYVYFYEP VNVEYFAMNN WSFLQSGSIG IDRKDIEKVF TGRTVLSSIY QDQRTKQNVM SLLTPVYVAG QLKGIVLLDI NKNNLRNIFY THDRPLLWRF LNVTLTDTDS GRDIIINQSE DNLFQYVSYV HDLPGGIRVS LSIDILYFIT SSWKSVLFWI LTALILLNMV RMHFRLYQNV SRENISDAMT GLYNRKILTP ELEQRLQKLV QSGSSVMFIA IDMDKLKQIN DTLGHQEGDL AITLLAQAIK QSIRKSDYAI RLGGDEFCII LVDSTPQIAA QLPERIEKRL QHIAPQKEIG FSSGIYAMKE NDTLHDAYKA SDERLYVNKQ NKNSRS. It is sometimes possible for the material contained within the vial of "Putative diguanylate cyclase YeaJ (yeaJ), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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