Nuclease-sensitive element-binding protein 1 Recombinant Protein | YBOX1 recombinant protein

Recombinant mouse Nuclease-sensitive element-binding protein 1

Cat. Num. AAA9422554

• Gene Names: Ybx1; EF1A; MSY1; YB-1; dbpB; Nsep1; C79409; mYB-1a; 1700102N10Rik
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Nuclease-sensitive element-binding protein 1; Recombinant mouse Nuclease-sensitive element-binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-ADNA-binding protein B; DBPBEnhancer factor I subunit A; EFI-AY-box transcription factorY-box-binding protein 1; YB-1; YBOX1 recombinant protein

• Host: Yeast
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 2-322aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for YBOX1 recombinant protein

Mediates pre-mRNA alternative splicing regulation. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. Regulates the transcription of numerous genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds preferentially to 5'-[CU]CUGCG-3' motif in vitro.The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 113205059
• NCBI GeneID: 22608
• NCBI Accession #: NP_035862.2
• NCBI GenBank Nucleotide #: NM_011732.2
• UniProt Accession #: P62960; P22568; P27817; P43482
• Molecular Weight: 37.6kD
• NCBI Official Full Name: nuclease-sensitive element-binding protein 1
• NCBI Official Synonym Full Names: Y box protein 1
• NCBI Official Symbol: Ybx1
• NCBI Official Synonym Symbols: EF1A; MSY1; YB-1; dbpB; Nsep1; C79409; mYB-1a; 1700102N10Rik
• NCBI Protein Information: nuclease-sensitive element-binding protein 1
• UniProt Protein Name: Nuclease-sensitive element-binding protein 1
• Protein Family: Nuclease-sensitive element-binding protein
• UniProt Gene Name: Ybx1
• UniProt Synonym Gene Names: Msy-1; Msy1; Nsep1; Yb1; CBF-A; DBPB; EFI-A; YB-1

Uniprot Description

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood.

Protein type: RNA processing; RNA splicing; RNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4|4 D2.1

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; intracellular membrane-bound organelle; nuclear membrane; nucleus; perinuclear region of cytoplasm; ribonucleoprotein complex; stress granule; U12-dependent spliceosome

Molecular Function: chromatin binding; DNA binding; GTPase binding; mRNA binding; p53 binding; protein binding; RNA binding; sequence-specific DNA binding; single-stranded DNA binding; transcription factor binding

Biological Process: in utero embryonic development; negative regulation of apoptosis; negative regulation of insulin receptor signaling pathway; negative regulation of striated muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent