X-ray repair cross-complementing protein 5 Recombinant Protein | XRCC5 recombinant protein

Recombinant Human X-ray repair cross-complementing protein 5

Cat. Num. AAA9420810

• Gene Names: XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: X-ray repair cross-complementing protein 5; Recombinant Human X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2ATP-dependent DNA helicase II 80 kDa subunitCTC box-binding factor 85 kDa subunit; CTC85; CTCBFDNA repair protein XR; CC5Ku80Ku86Lupus Ku autoantigen protein p86Nuclear factor IVThyroid-lupus autoantigen; TLAAX-ray repair complementin; XRCC5 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Partial, 251-455aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for XRCC5 recombinant protein

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V (D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing th together. The assbly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 10863945
• NCBI GeneID: 7520
• NCBI Accession #: NP_066964.1
• NCBI GenBank Nucleotide #: NM_021141.3
• UniProt Accession #: P13010; Q0Z7V0; Q4VBQ5; Q53HH7; Q7M4N0; Q9UCQ0; Q9UCQ1; A8K3X5
• Molecular Weight: 27.53kD
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair cross complementing 5
• NCBI Official Symbol: XRCC5
• NCBI Official Synonym Symbols: KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
• NCBI Protein Information: X-ray repair cross-complementing protein 5
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• Protein Family: X-ray repair cross-complementing protein
• UniProt Gene Name: XRCC5
• UniProt Synonym Gene Names: G22P2; CTC85; CTCBF; TLAA

NCBI Description

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Uniprot Description

Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.

Protein type: DNA-binding; EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cytosol; extracellular region; membrane; nuclear chromosome, telomeric region; nuclear telomere cap complex; nucleoplasm; nucleus; plasma membrane; protein complex

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; double-stranded DNA binding; double-stranded telomeric DNA binding; enzyme activator activity; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding; ubiquitin protein ligase binding

Biological Process: double-strand break repair; double-strand break repair via nonhomologous end joining; negative regulation of transcription, DNA-dependent; neutrophil degranulation; positive regulation of catalytic activity; positive regulation of interferon type I production; positive regulation of protein kinase activity; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; regulation of smooth muscle cell proliferation; regulation of telomere maintenance